Serological and Virological Investigation of the Role of the Herpesviruses EBV, CMV and HHV-6 in Post-Infective Fatigue Syndrome
Serological and Virological Investigation of the Role of the Herpesviruses EBV, CMV and HHV-6 in Post-Infective Fatigue Syndrome
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DOI：
10.1002/jmv.21873
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发表时间：
2010-10-01
影响因子：
12.7
通讯作者：
Lloyd, Andrew
中科院分区：
文献类型：
--
作者：
Cameron, Barbara;Flamand, Louis;Lloyd, Andrew
多个先前的研究已经寻找了慢性疲劳综合征（CFS）患者中疱疹病毒持续、活动性感染或再激活的证据，结果相互矛盾。本研究旨在通过研究20例CFS发病和演变的良好特征模型（杜博感染结局研究（DIOS）的前瞻性队列）来阐明这一点。选择进行检查的患者包括5例原发性EB病毒（EBV）感染的CFS患者; 5例非EBV引起的急性病毒感染的CFS患者;和10例原发性EBV感染迅速消退的匹配对照。使用一系列针对EBV、HHV-6和CMV的血清学检测对三个时间点的血清样本进行检测。使用定量PCR测定法评估病毒基因组。所有患者HHV-6血清阳性，10例CMV感染基线血清阳性（5例患者和5例对照）。低滴度CMV IgM抗体被发现在感染基线在这些病例中的两个和三个对照患者。HHV-6 IgG抗体滴度在感染基线时最高，但在CFS病例和对照患者之间没有差异。随着时间的推移，EBV IgG VCA p18，EBNA-1 IgG和EA IgG滴度增加，但这些在CFS病例和对照患者之间没有差异。在少数样品中EBV和HHV 6 DNA水平处于对照水平，并且在所有样品中均检测不到CMV。这些数据不支持持续或再激活的EBV、HHV-6或CMV感染在CFS发病机制中的假设。J. Med. Virol. 82：1684-1688，2010. (C)2010 Wiley-Liss，Inc.
Multiple previous studies have sought evidence for ongoing, active infection with, or reactivation of, Herpesviruses in patients with chronic fatigue syndrome (CFS), with conflicting results. This study aimed to clarify this by studying 20 patients enrolled in a well-characterized model of the onset and evolution of CFS, the prospective cohort of the Dubbo Infection Outcomes Study (DIOS). The patients selected for examination included five CFS patients with primary Epstein-Barr virus (EBV) infection; five CFS patients with acute viral infection not caused by EBV; and 10 matched controls with prompt resolution of primary EBV infection. Serum samples from three timepoints were assayed using a comprehensive range of serological assays for EBV, HHV-6, and CMV. Viral genomes were assessed using quantitative PCR assays. All patients were seropositive for HHV-6, and 10 were seropositive for CMV at infection baseline (five patients and five controls). Low titer CMV IgM antibodies were found at infection baseline in two of these cases and three control patients. HHV-6IgG antibody titers were highest at infection baseline but did not differ between the CFS cases and the control patients. There were increases in EBV IgG VCA p18, EBNA-1 IgG, and EA IgG titers over time, but these did not differ between CFS cases and control patients. EBV and HHV6 DNA levels were at control levels in a minority of samples, and CMV was undetectable in all samples. These data do not support the hypothesis of ongoing or reactivated EBV, HHV-6, or CMV infection in the pathogenesis of CFS. J. Med. Virol. 82:1684-1688, 2010. (C) 2010 Wiley-Liss, Inc.