C9orf10 protein, a novel protein component of Purα-containing mRNA-protein particles (Purα-mRNPs):: Characterization of developmental and regional expressions in the mouse brain
C9orf10 protein, a novel protein component of Purα-containing mRNA-protein particles (Purα-mRNPs):: Characterization of developmental and regional expressions in the mouse brain
复制标题
DOI：
10.1369/jhc.2008.950733
复制
发表时间：
2008-08-01
影响因子：
3.2
通讯作者：
Anzai, Kaijiro
中科院分区：
文献类型：
--
作者：
Kobayashi, Yuriko;Suzuki, Keiichi;Anzai, Kaijiro
PURα参与了神经元中的mRNA转运和翻译。我们以前报道过Purα是与其他几种蛋白质形成的mRNA/蛋白质复合体(Purα-mRNPs)的一个组成部分。其中，我们发现了C9orf10(人类9号染色体开放阅读框架10)蛋白，该蛋白最近被鉴定为含有RNA的结构的组成部分。然而，C9orf10本身仍然知之甚少。为了在蛋白质水平上表征C9orf10的表达，我们提出了一种针对C9orf10的抗体，并比较了该蛋白和purα在小鼠脑中的空间和发育表达。C9orf10在胚胎期12就有表达，而Pura在出生后5天就有表达。在成人中，C9orf10的表达主要集中在海马区、尾壳核、大脑皮层和小脑，与Pura的均匀分布不同。C9orf10阳性细胞对Purα也呈免疫反应。根据对神经元特异性核蛋白或CaM激酶II的免疫反应性判断，C9orf10的表达仅限于神经元。这些观察表明，C9orf10对Pura在有限的脑区起辅助作用，以及其他尚未确定的可能功能。
Pur alpha has been implicated in mRNA transport and translation in neurons. We previously reported that Pur alpha is a component of mRNA/protein complexes (Pur alpha-mRNPs) with several other proteins. Among them, we found the C9orf10 (Homo sapiens chromosome 9 open reading frame 10) protein, which was recently characterized as a component of RNA-containing structures. However, C9orf10 itself remains poorly understood. To characterize C9orf10 expression at the protein level, we raised an antibody against C9orf10 and compared the spatial and developmental expressions of this protein and Pur alpha in the mouse brain. C9orf10 was expressed as early as embryo stage 12, whereas Pura was expressed from 5 days after birth. In adults, C9orf10 expression was most prominent in the hippocampus, caudate putamen, cerebral cortex, and cerebellum, unlike the uniform distribution of Pura. C9orf10-positive cells also showed immunoreactivity to Pur alpha. C9orf10 expression was restricted to neurons, judging by the immunoreactivity to neuron-specific nuclear protein or CaM kinase II. These observations suggest an accessory role of C9orf10 for Pura in a limited brain region in addition to other possible functions that have not yet been determined.