Enterovirus type 71 neutralizing antibodies in the serum of macaque monkeys immunized with EV71 virus-like particles
Enterovirus type 71 neutralizing antibodies in the serum of macaque monkeys immunized with EV71 virus-like particles
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DOI：
10.1016/j.vaccine.2011.12.081
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发表时间：
2012-02-08
期刊：
影响因子：
5.5
通讯作者：
Chiang, Bor-Luen
中科院分区：
文献类型：
--
作者：
Lin, Yu-Li;Yu, Chun-I;Chiang, Bor-Luen
71 型肠道病毒 (EV71) 是一种剧毒肠道病毒，会导致三岁以下儿童住院。目前没有针对 EV71 的抗病毒疗法或疫苗。由于脊髓灰质炎样瘫痪和致命性脑炎的风险很高，有效的 EV71 疫苗有可能预防病毒引起的发病率和死亡率。在这项研究中，我们首先测试了一种基于病毒样颗粒 (VLP) 的潜在 EV71 候选疫苗。我们对猕猴进行了疫苗接种，以验证 VLP 疫苗对 EV71 的免疫原性。我们检测了 VLP 或 EV71 特异性抗体、中和滴度、ELISPOT 和 T 细胞反应，以发现它们对 EV71 的免疫反应。当将明矾佐剂的 VLP 疫苗给予猕猴时，这些猴子对 EV71 产生了特异性体液和细胞免疫反应。尽管在接受 VLP 免疫的猴子血清中发现的 EV71 中和抗体低于接种灭活 EV71 疫苗的猴子，但基于 VLP 的疫苗产生了针对 EV71 的记忆免疫反应。因此，基于VLP的EV71疫苗是针对EV71感染的潜在疫苗。 (C) 2012 Elsevier Ltd. 保留所有权利。
Enterovirus type 71 (EV71) is a virulent form of enteroviruses causing hospitalizations for children less than three years of age. Currently there are no anti-viral therapies or vaccines available for EV71. Due to the high risk of poliomyelitis-like paralysis and fatal encephalitis, an effective vaccine to EV71 could potentially prevent virus-induced morbidity and mortality. In this study, we first tested a potential EV71 vaccine candidate based on virus-like particles (VLP). We vaccinated macaque monkeys to validate the immunogenicity of the VLP vaccine to EV71. We detected the VLP or EV71-specific antibodies, neutralization titers, ELISPOT, and T cell response to find their immune responses to EV71. When the VLP vaccine adjuvanted with alum was given to macaque monkeys, these monkeys developed both specific humoral and cellular immune responses to EV71. Despite lower neutralizing antibodies to EV71 were found in sera of VLP-immunized monkeys than monkeys vaccinated with inactivated EV71, VLP-based vaccine generated a memory immune response to EV71. Hence, VLP-based EV71 vaccine is a potential vaccine against EV71 infection. (C) 2012 Elsevier Ltd. All rights reserved.