All-Aqueous Assemblies via Interfacial Complexation: Toward Artificial Cell and Microniche Development
All-Aqueous Assemblies via Interfacial Complexation: Toward Artificial Cell and Microniche Development
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DOI：
10.1021/acs.langmuir.7b02237
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发表时间：
2017-10-03
期刊：
影响因子：
3.9
通讯作者：
Lee, Daeyeon
中科院分区：
文献类型：
--
作者：
Hann, Sarah D.;Stebe, Kathleen J.;Lee, Daeyeon
在自然界中，生物分子和活细胞周围的环境可以决定它们的结构、功能和性质。禁闭是界定和管理这种环境的一个关键手段。例如，将适当的成分限制在隔室中有利于生化机器以及亚细胞器的组装、动力学和功能。特别是无膜细胞器，被认为是通过细胞内部空间内定义的热力学线索形成的。在更大的长度尺度上，活细胞的限制决定了哺乳动物和细菌细胞的细胞功能。一类有前途的人工结构，可以概括这些多尺度限制效应是基于水两相系统（ATPS）。这篇专题文章重点介绍了ATPS液滴体系的生产和稳定化方面的最新进展，重点是界面络合。这些系统能够实现结构形成、调制和触发（分解）组装，从而允许结构被定制以适合所需功能并被设计用于特定限制研究。确定了合成细胞和利基研究的开放问题。
In nature, the environment surrounding biomolecules and living cells can dictate their structure, function, and properties. Confinement is a key means to define and regulate such environments. For example, the confinement of appropriate constituents in compartments facilitates the assembly, dynamics, and function of biochemical machineries as well as subcellular organelles. Membraneless organelles, in particular, are thought to form via thermodynamic cues defined within the interior space of cells. On larger length scales, the confinement of living cells dictates cellular function for both mammalian and bacterial cells. One promising class of artificial structures that can recapitulate these multiscale confinement effects is based on aqueous two-phase systems (ATPSs). This feature article highlights recent developments in the production and stabilization of ATPS-droplet-based systems, with a focus on interfacial complexation. These systems enable structure formation, modulation, and triggered (dis)assembly, thereby allowing structures to be tailored to fit the desired function and designed for particular confinement studies. Open issues for both synthetic cells and niche studies are identified.