An organic thin film photodiode as a portable photodetector for the detection of alkylphenol polyethoxylates by a flow fluorescence-immunoassay on magnetic microbeads in a microchannel
An organic thin film photodiode as a portable photodetector for the detection of alkylphenol polyethoxylates by a flow fluorescence-immunoassay on magnetic microbeads in a microchannel
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DOI：
10.1016/j.talanta.2013.08.044
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发表时间：
2013-12-15
期刊：
影响因子：
6.1
通讯作者：
Imato, Toshihiko
中科院分区：
文献类型：
--
作者：
Ishimatsu, Ryoichi;Naruse, Azusa;Imato, Toshihiko
将有机薄膜光电二极管（OPD）作为便携式光电探测器，成功地应用于非离子表面活性剂烷基酚聚氧乙烯醚（APnEOs）的竞争酶联免疫吸附测定（ELISA）中。在测定中使用用抗APnEO抗体化学固定的微珠。OPD由铜酞菁（CuPc）、C-60的层和浴铜灵（BCP）的第二层组成，所述浴铜灵（BCP）具有由CuPc和Ca-60组成的本体异质结，所述本体异质结通过气相沉积方法在氧化铟锡涂覆的玻璃基底上制备。OPD显示对于波长为585 nm的光的入射光子电流效率（IPCE）为约19%。在585 nm处的这种相对高的IPCE使其适合于检测试卤灵的荧光（λ（em）=585 nm），试卤灵是通过Amplex Red与辣根过氧化物酶（HRP）和H2 O2的酶促反应产生的竞争性ELISA的产物。通过将制造的OPD和商业LED分别作为光电探测器和光源，将荧光检测器组装在微芯片上。在0至8 μ M的浓度范围内，由于试卤灵的荧光而产生的OPD光电流与试卤灵的浓度成正比。当所制造的OPD被用作便携式光电检测器时，使用HRP标记的APnEOs（HRP-APnEOs）在其表面已固定有抗APnEOs抗体的磁性微珠上进行APnEOs的竞争性ELISA。获得了典型的S形校准曲线，并且数据与数值模拟非常一致，其中OPD的光电流相对于通过竞争性ELISA测定的APnEO浓度作图。在批处理和流动系统中，APnEO免疫测定的检测限分别约为2和4 ppb。(C)2013爱思唯尔有限公司版权所有。
An organic thin film photodiode (OPD) was successfully employed as a portable photodetector in a competitive enzyme-linked immunosorbent assay (ELISA) of a class of nonionic surfactants, namely alkylphenol polyethoxylates (APnEOs) which are an environmental pollutant. Microbeads that were chemically immobilized with an anti-APnEOs antibody were used in the assay. The OPD consisted of a layer of copper phthalocyanine (CuPc), C-60 and a second layer of bathocuproine (BCP) with a bulk heterojunction composed of CuPc and Ca-60 prepared by a vapor deposition method on an indium-tin oxide coated glass substrate. The OPD showed an incident photon-current efficiency (IPCE) of approximately 19% for light at a wavelength of 585 nm. This relatively high IPCE at 585 nm makes it suitable for detecting the fluorescence of resorufin (lambda(em)=585 nm), the product of the competitive ELISA, produced through the enzymatic reaction of Amplex Red with horseradish peroxidase (HRP) and H2O2. A fluorometric detector was assembled on a microchip by combining the fabricated OPD and a commercial LED as a photodetector and a light source, respectively. The photocurrent of the OPD due to the fluorescence of resorufin was proportional to the concentration of resorufin in the concentration range from 0 to 8 mu M. When the fabricated OPD was used as a portable photodetector, the competitive ELISA of APnEOs using HRP labeled APnEOs (HRP-APnEOs) was performed on magnetic microbeads on which surface an anti-APnEOs antibody had been immobilized. A typical sigmoidal calibration curve was obtained and the data were in good agreement with a numerical simulation, where the photocurrent of the OPD was plotted against the concentration of APnEOs, determined via the competitive ELISA. The detection limit of the immunoassay for APnEOs was approximately 2 and 4 ppb in batch and flow system, respectively. (C) 2013 Elsevier B.V. All rights reserved.