CONTROL ELEMENTS OF THE DROSOPHILA SEGMENTATION GENE FUSHI-TARAZU
CONTROL ELEMENTS OF THE DROSOPHILA SEGMENTATION GENE FUSHI-TARAZU
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DOI：
10.1016/0092-8674(85)90232-6
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发表时间：
1985-12-01
期刊：
影响因子：
64.5
通讯作者：
GEHRING, WJ
中科院分区：
文献类型：
--
作者：
HIROMI, Y;KUROIWA, A;GEHRING, WJ
果蝇Fushi Tarazu(FTZ)是果蝇建立节段模式所需的同源盒基因之一。FTZ+转录本在胚胎期积累在细胞中，形成七条等间距的条带。我们通过胚系转化的方法分析了FTZ基因的顺式作用调控序列。功能性FTZ基因包括一个1.9kb和.apprx的转录单位。6.1kb的5‘-侧翼序列。将5‘-’侧翼序列与大肠杆菌LacZ基因融合，可在转化胚胎中以斑马模式引导β-半乳糖苷酶的表达。融合基因也在某些神经母细胞中表达，这些神经母细胞对所有节段的神经节细胞都有贡献。在5‘-侧翼序列中已经发现了各种形态发生控制元件。
Fushi tarazu (ftz) is one of the Drosophila homeo-box-containing genes required to establish the segmental pattern. Ftz+ transcripts accumulate in cells that form seven equally spaced bands at the blastoderm stage. We have analyzed cis-acting controlling sequences of the ftz gene by germ line transformation. The functional ftz gene includes a transcription unit of 1.9 kb and .apprx. 6.1 kb of the 5''-flanking sequences. Fusion of the 5''-flanking sequences to the Escherichia coli lacZ gene directs expression of .beta.-galactosidase in a "zebra" pattern in transformed embryos. The fusion gene is also expressed in certain neuroblasts that contribute to ganglion cells of all segments. Various morphogenetic controlling elements have been identified within the 5''-flanking sequences.