Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets
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DOI：
10.1128/jcm.01780-09
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发表时间：
2010-04-01
影响因子：
9.4
通讯作者：
Greub, Gilbert
中科院分区：
文献类型：
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作者：
Prod'hom, Guy;Bizzini, Alain;Greub, Gilbert
使用氯化铵红细胞裂解程序从阳性血培养物中制备细菌沉淀，用于直接基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析。对78.7%的供试颗粒进行了鉴别。此外，考虑有效分数时，99%的MALDI-TOF鉴定在物种水平上一致。这种快速准确的方法是有前途的。
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.