The Helicobacter pylori duodenal ulcer promoting gene, dupA in China.
The Helicobacter pylori duodenal ulcer promoting gene, dupA in China.
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中国幽门螺杆菌十二指肠溃疡促基因dupA
DOI：
10.1186/1471-230x-8-49
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发表时间：
2008-10-25
影响因子：
2.4
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Zhang Z;Zheng Q;Chen X;Xiao S;Liu W;Lu H
H. pylori在中国人群中高达60-70%。虽然十二指肠溃疡和胃癌都是由H.幽门螺杆菌，他们是在光谱的两端，因此被认为是相互排斥的。十二指肠溃疡促进基因（dupA）与十二指肠溃疡的发生有关。本研究旨在检测幽门螺杆菌（Helicobacter pylori，Hp）dupA基因在不同胃十二指肠疾病患者中的分布情况，并探讨该基因与其他毒力因子的关系。H.幽门螺杆菌分离自慢性胃炎、十二指肠溃疡（DU）、胃溃疡（GU）或非贲门胃癌患者的胃活检。聚合酶链反应检测dupA、cagA、vacA、iceA和babA 2基因型。胃粘膜活检标本的组织学特征进行了分级的基础上提出的评分系统的更新悉尼系统。采用限制性片段长度多态性（RFLP）分析IL-1β基因多态性。对360例患者（包括133例慢性胃炎、101例DU、47例GU和79例非贲门胃癌）的胃粘膜进行了检测。dupA基因阳性率为35.3%（127/360），DU组dupA基因阳性率为45.5%，显著高于胃癌组和GU组（24.1%和23.4%）（P < 0.05）。感染dupA阳性菌株的患者的慢性炎症评分高于感染dupA阴性菌株的患者（2.36 vs. 2.24，p = 0.058）。dupA的存在与cagA、vacA、iceA和babA 2基因型或IL 1β多态性无关。在中国，dupA基因在DU中的患病率最高，与GU和胃癌呈负相关。
The prevalence of H. pylori is as high as 60–70% in Chinese population. Although duodenal ulcer and gastric cancer are both caused by H. pylori, they are at opposite ends of the spectrum and as such are considered mutually exclusive. Duodenal ulcer promoting (dupA) gene was reported to be associated with duodenal ulcer development. The aim of this study was to determine the prevalence of dupA gene of Helicobacter pylori in patients with various gastroduodenal diseases and to explore the association between the gene and other virulence factors. H. pylori were isolated from gastric biopsies of patients with chronic gastritis, duodenal ulcer (DU), gastric ulcer (GU), or non-cardia gastric carcinoma. The dupA, cagA, vacA, iceA and babA2 genotypes were determined by polymerase chain reaction. Histological features of gastric mucosal biopsy specimens were graded based on the scoring system proposed by the updated Sydney system. IL-1β polymorphism was investigated using restriction fragment length polymorphism. Isolates from 360 patients including 133 with chronic gastritis, 101 with DU, 47 with GU, and 79 with non-cardia gastric carcinoma were examined. The dupA gene was detected in 35.3% (127/360) and the prevalence DU patients was significantly greater than that in gastric cancer or GU patients (45.5% vs. 24.1% and 23.4%, P < 0.05). Patients infected with dupA-positive strains had higher scores for chronic inflammation compared to those with dupA-negative strains (2.36 vs. 2.24, p = 0.058). The presence of dupA was not associated with the cagA, vacA, iceA and babA 2 genotypes or with IL-1β polymorphisms. In China the prevalence of dupA gene was highest in DU and inversely related to GU and gastric cancer.