Apparent co-sequestration of immunoreactive corticotropin, alpha-melanotropin, and gamma-lipotropin in hypothalamic granules.
Apparent co-sequestration of immunoreactive corticotropin, alpha-melanotropin, and gamma-lipotropin in hypothalamic granules.
复制标题
下丘脑颗粒中免疫反应性促肾上腺皮质激素、α-促黑素和γ-促脂激素的明显共螯合。
DOI：
10.1111/j.1471-4159.1981.tb01703.x
复制
发表时间：
1981
影响因子：
4.7
通讯作者：
Porter,JC
中科院分区：
文献类型：
--
作者：
Barnea,A;Cho,G;Porter,JC
在本研究中，解决了免疫反应性α-促黑素激素（α-MSH 1）、促肾上腺皮质激素（ACTH 1）和β-促黑素激素（β-MSH 1）是否共同螯合在成年雄性大鼠下丘脑颗粒中的问题。当在非平衡条件下，在连续蔗糖密度梯度上分离由下丘脑匀浆制备的900 × g上清液时，观察到含有α-MSH 1、ACTH 1或β-MSH 1的两个颗粒群。然而，当在平衡条件下分离时，含有α-MSH 1ACTH 1或β-MSH 1的两种颗粒群作为单一条带回收。这种沉降特性表明，含有给定肽的颗粒大小不同，但密度相似。在它们的沉降中，含有α-MSH 1、ACTH 1和β-MSH 1的小颗粒与含有α-MSH 1的颗粒难以区分，而含有α-MSH 1的大颗粒（ACTH 1和β-MSH 1）与含有α-MSH 1的突触体难以区分，β-MSH 1具有表观分子量（M.W.）大约5,000，这与γ-促脂素相似。ACTH 1由三种分子组成：大分子（M.W.≥ 10，000）、5.7K（M.W. 5，700）和4.5K（M.W. 4，500英镑）。大ACTH为主要成分，5.7KACTH为次要成分，存在于颗粒和突触体中。这些结果表明，α-MSH、ACTH及其前体和γ-促脂素在下丘脑颗粒中共螯合。
In the present study, the question of whether immunoreactive α‐melanotropin (α‐MSH1), corticotropin (ACTH1), and β‐melanotropin (β‐MSH1) are co‐sequestered in hypothalamic granules of adult male rats was addressed. When a 900 ×gsupernatant fluid prepared from a hypothalamic homogenate was fractionated on continuous sucrose density gradients under non‐equilibrium Conditions, two populations of particles containing α‐MSH1, ACTH1, or β‐MSH1Were observed. However, when fractionated under equilibrium conditions, the two populations of particles containing α‐MSH1ACTH1, or β‐MSH1were recovered as a single band. This sedimentation characteristic indicates that the particles containing a given peptide differ in size but are similar in density. In their sedimentation, the small particles containing α‐MSH1, ACTH1, and β‐MSH1are indistinguishable from granules containing α‐MSH1, whereas the large particles containing α‐MSH1(ACTH1, and β‐MSH1are indistinguishable from synaptosomes containing α‐MSH1, β‐MSH1had an apparent molecular weight (M.W.) of about 5,000, which is similar to that of γ‐lipotropin. ACTH1was comprised of three species of molecules: big (M.W. ≥ 10,000), 5.7K (M.W. ≌ 5,700), and 4.5K (M.W. ≌ 4,500). Big ACTH was the predominant and 5.7K ACTH the minor component of ACTH1present in granules as well as in synaptosomes. These results are suggestive that α‐MSH, ACTH and its precursors, and γ‐lipotropin are co‐sequestered in hypothalamic granules.