NUCLEAR MATRIX PROTEINS REFLECT CELL TYPE OF ORIGIN IN CULTURED HUMAN-CELLS
NUCLEAR MATRIX PROTEINS REFLECT CELL TYPE OF ORIGIN IN CULTURED HUMAN-CELLS
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DOI：
10.1073/pnas.85.1.121
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发表时间：
1988-01-01
影响因子：
11.1
通讯作者：
PENMAN, S
中科院分区：
文献类型：
--
作者：
FEY, EG;PENMAN, S
将核基质（NM）的低丰度蛋白与中间丝（IF）蛋白分离，并通过二维凝胶电泳进行分析。三个人乳腺癌细胞系具有几乎相同的37个NM蛋白的模式。相反，来自不同组织的细胞系具有不同的NM蛋白质模式。总之，这里检查的五种细胞类型共有205种可区分的NM蛋白，其中125种蛋白是单一细胞类型所特有的。其余NM蛋白在不同细胞类型之间有不同程度的共享。多克隆抗血清，通过免疫获得的总NM蛋白作为抗原，优先染色的核内部，而不是外部IF。这些观察结果表明，NM蛋白，定位于细胞核的内部，以细胞类型特异性的方式变化。
The low abundance proteins of the nuclear matrix (NM) were separated from the intermediate filament (IF) proteins and analyzed by two-dimensional gel electrophoresis. Three human breast carcinoma lines had virtually identical patterns of 37 NM proteins. In contrast, cell lines derived from diverse tissues had qualitatively different NM protein patterns. Together, the five cell types examined here had a total of 205 distinguishable NM proteins with 125 of these proteins unique to a single cell type. The remaining NM proteins were shared among cell types to different degrees. Polyclonal antisera, obtained by immunization with total NM proteins as antigens, preferentially stained the nuclear interior and not the exterior IF. These observations suggest that the NM proteins, localized to the interior of the nucleus, vary in a cell-type-specific manner.