Osteochondral defect repair with autologous bone marrow-derived mesenchymal stem cells in an injectable, in situ, cross-linked synthetic extracellular matrix
Osteochondral defect repair with autologous bone marrow-derived mesenchymal stem cells in an injectable, in situ, cross-linked synthetic extracellular matrix
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DOI：
10.1089/ten.2006.12.3405
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发表时间：
2006-12-01
影响因子：
--
通讯作者：
Prestwich, Glenn D.
中科院分区：
文献类型：
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作者：
Liu, Yanchun;Shu, Xiao Zheng;Prestwich, Glenn D.
将化学修饰的透明质酸和明胶组成的共交联合成细胞外基质（sECM）用作兔模型中骨软骨缺损修复的细胞递送载体。在2只兔关节的股骨关节软骨的髌骨沟中产生全层缺损，并分配4个实验组（12只兔/组）：未处理对照、仅自体间充质干细胞（MSC）、仅sECM和MSC + sECM。使sECM水凝胶在缺损处原位交联。在术后4、8和12周处死家兔，并对软骨修复进行评价和评分。在对照组中，缺损填充纤维组织。单纯MSC组，透明样软骨填充缺损周边区域，但中心填充纤维组织。在仅sECM组中，具有带状结构的透明软骨在12周时填充了缺损，但修复的软骨与相邻宿主软骨之间的界面是明显的。在MSC + sECM组中，缺损在12周时被弹性、坚固、半透明的软骨完全填充，并且显示修复组织与正常软骨的上级整合。sECM可以输送和保留MSC，并且可注射的细胞接种的sECM可以在临床上通过关节镜输送。
A co-cross-linked synthetic extracellular matrix (sECM) composed of chemically modified hyaluronic acid and gelatin was used as a cell delivery vehicle for osteochondral defect repair in a rabbit model. A full-thickness defect was created in the patellar groove of the femoral articular cartilage in each of 2 rabbit joints, and 4 experimental groups were assigned ( 12 rabbits/group): untreated control, autologous mesenchymal stem cells (MSCs) only, sECM only, and MSCs + sECM. The sECM hydrogels were allowed to cross-link in the defect in situ. Rabbits were sacrificed at 4, 8, and 12 weeks post-surgery, and cartilage repair was evaluated and scored. In the controls, defects were filled with fibrous tissue. In the MSC-only group, hyaline-like cartilage filled the peripheral area of the defect, but the center was filled with fibrous tissue. In the sECM-only group, hyaline cartilage with zonal architecture filled the defect at 12 weeks, but an interface between repaired and adjacent host cartilage was evident. In the MSCs + sECM group, defects were completely filled with elastic, firm, translucent cartilage at 12 weeks and showed superior integration of the repair tissue with the normal cartilage. The sECM delivers and retains MSCs, and the injectable cell-seeded sECM could be delivered arthroscopically in the clinic.