A multiphase transitioning peptide hydrogel for suturing ultrasmall vessels.
A multiphase transitioning peptide hydrogel for suturing ultrasmall vessels.
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DOI：
10.1038/nnano.2015.238
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发表时间：
2016-01
影响因子：
38.3
通讯作者：
Schneider JP
中科院分区：
文献类型：
--
作者：
Smith DJ;Brat GA;Medina SH;Tong D;Huang Y;Grahammer J;Furtmüller GJ;Oh BC;Nagy-Smith KJ;Walczak P;Brandacher G;Schneider JP
许多手术因需要吻合或重新连接微米级的血管而变得复杂。尽管缝合仍然是吻合血管的黄金标准，但很难通过塌陷的管腔正确地放置缝线，使该过程容易失败。在这里，我们报告了一种多相转变多肽水凝胶，可以注射到血管的管腔内，以促进缝合。这种含有光笼谷氨酸的多肽在注射器中形成一种固体状凝胶，可以剪切薄地传递到收缩的血管的管腔(在那里它扩张血管)，以及两条血管之间的空间(在那里它被用来接近血管的末端)。缝合是直接通过凝胶进行的。光被用来启动最终的凝胶-溶胶相变，破坏水凝胶网络，使凝胶被移除，血液流动恢复。这种凝胶为微型和超显微外科手术增加了一种新的工具。
Many surgeries are complicated by the need to anastomose, or reconnect, micron-scale vessels. Although suturing remains the gold standard for anastomosing vessels, it is difficult to place sutures correctly through collapsed lumen, making the procedure prone to failure. Here, we report a multi-phase transitioning peptide hydrogel that can be injected into the lumen of vessels to facilitate suturing. The peptide, which contains a photocaged glutamic acid, forms a solid-like gel in a syringe and can be shear-thin delivered to the lumen of collapsed vessels (where it distends the vessel), and the space between two vessels (where it is used to approximate the vessel ends). Suturing is performed directly through the gel. Light is used to initiate the final gel-sol phase transition that disrupts the hydrogel network, allowing the gel to be removed and blood flow to resume. This gel adds a new tool to the armamentarium for micro- and supermicrosurgical procedures.