THE GAL(ALPHA-1-4)GAL-SPECIFIC TIP ADHESIN OF ESCHERICHIA-COLI P-FIMBRIAE IS NEEDED FOR PYELONEPHRITIS TO OCCUR IN THE NORMAL URINARY
THE GAL(ALPHA-1-4)GAL-SPECIFIC TIP ADHESIN OF ESCHERICHIA-COLI P-FIMBRIAE IS NEEDED FOR PYELONEPHRITIS TO OCCUR IN THE NORMAL URINARY
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DOI：
10.1073/pnas.91.25.11889
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发表时间：
1994-12-06
影响因子：
11.1
通讯作者：
NORMARK, S
中科院分区：
文献类型：
--
作者：
ROBERTS, JA;MARKLUND, BI;NORMARK, S
人类非梗阻性急性肾盂肾炎最常由p纤带大肠杆菌引起。p -纤维是一种异聚纤维，其远端携带Gal(α 1-4)Gal特异性的PapG粘附素。肾盂肾炎菌株DS17从单个基因簇表达p -菌膜。通过在PapG基因早期引入1 bp缺失的等位基因置换，构建了表达缺乏PapG粘附素的p -菌毛的突变菌株DS17-8。在食食猴中，DS17和DS17-8同样能引起膀胱感染，而只有野生型菌株DS17能引起肾盂肾炎，通过细菌学、功能和组织病理学标准进行监测。由于DS17（而非DS17-8）粘附在肾组织上，这些数据强调了微生物粘附在宿主组织在感染性疾病中的关键作用，并强烈提示p -菌毛的PapG尖端粘附素在人类肾脏感染的发病机制中是必不可少的。
Nonobstructive acute pyelonephritis in humans is most often caused by P-fimbriated Escherichia coli. P-fimbriae are heteropolymeric fibers carrying a Gal(alpha 1-4)Gal-specific PapG adhesin at its distal end. The pyelonephritic strain DS17 expresses P-fimbriae from a single gene cluster. A mutant strain, DS17-8, which expresses P-fimbriae lacking the PapG adhesin, was constructed by allelic replacement introducing a 1-bp deletion early in the papG gene. In cynomolgus monkeys, DS17 and DS17-8 were equally able to cause bladder infection, whereas only the wild-type strain DS17 could cause pyelonephritis as monitored by bacteriological, functional and histopathological criteria. Since DS17, but not DS17-8, adheres to renal tissue, these data underscore the critical role of microbial adherence to host tissues in infectious disease and strongly suggest that the PapG tip adhesin of P-fimbriae is essential in the pathogenesis of human kidney infection.