Fusarium head blight resistance loci in a stratified population of wheat landraces and varieties
Fusarium head blight resistance loci in a stratified population of wheat landraces and varieties
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小麦地方品种和品种分层群体中的镰刀菌赤霉病抗性位点
DOI：
10.1007/s10681-015-1539-4
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发表时间：
2016-02-01
期刊：
影响因子：
1.9
通讯作者：
Gu, Shiliang
中科院分区：
文献类型：
--
作者：
Li, Tao;Zhang, Dadong;Gu, Shiliang
为了确定中国和日本小麦地方品种是否具有独特的赤霉病抗性来源，利用364个全基因组简单重复序列和序列标签位点(STS)标记对195份小麦材料(包括商品品种和地方品种)进行了联合作图，并在3个温室单花接种试验中对其进行了II型赤霉病抗性鉴定。种群结构分析将该种群分为5个群体，中国地方品种分为4个群体。51份中国地方种质中的32份和27份日本种质中的24份归为一类。使用混合模型的关联分析在至少两个实验中确定了11个与FHB抗性有显著关联的标记。这些标记大多与已知的赤霉病抗性数量性状基因座(QTL)相吻合，其中1个新的QTL可能与Xgdm138-5DS和Xgwm358-5DS相关。Xbarc19-3AS在三个试验中均达到显著水平，其有利等位基因频率均在53%以上。在大多数可重复的标记座位上，中国地方品种和日本材料具有更有利的等位基因。根据有利等位基因的数量，确定了9个QTL组合。平均FHB严重程度随着可重复座位上有利等位基因数目的减少而增加。这些抗病基因座将使小麦赤霉病抗性基因库进一步多样化，并为育种家提供更多的抗源以改良小麦对赤霉病的抗性。
To determine if Chinese and Japanese wheat landraces and varieties have unique sources of Fusarium head blight (FHB) resistance, an association mapping panel of 195 wheat accessions including both commercial varieties and landraces was genotyped with 364 genome-wide simple sequence repeat and sequence-tagged site (STS) markers, and evaluated for type II FHB resistance in three greenhouse experiments using single floret inoculation. Population structure analysis stratified this population into five groups with Chinese landraces in four groups. Thirty-two of 51 Chinese landraces and 24 of 27 Japanese accessions were placed in one group. Association analysis using a mixed model identified 11 markers having significant associations with FHB resistance in at least two experiments. Most of these markers coincided with known quantitative trait loci (QTL) for FHB resistance with one potentially novel QTL associated with Xgdm138-5DS and Xgwm358-5DS. Xbarc19-3AS was significant in all three experiments, and the frequency of favorable alleles was more than 53 %. Chinese landraces and Japanese accessions had more favorable alleles at the majority of reproducible marker loci. Nine QTL combinations were identified according to the number of favorable alleles. Mean FHB severities increased with decreasing numbers of favorable alleles at reproducible loci. The resistance loci characterized here will further diversify the wheat FHB resistance gene pool, and provide breeders with additional sources of resistance for improvement of FHB resistance in wheat.