Impact of bioaccessible pyrene on the abundance of antibiotic resistance genes during Sphingobium sp.- and sophorolipid-enhanced bioremediation in soil
Impact of bioaccessible pyrene on the abundance of antibiotic resistance genes during Sphingobium sp.- and sophorolipid-enhanced bioremediation in soil
复制标题
土壤中鞘氨醇和槐糖脂增强生物修复过程中生物可利用芘对抗生素抗性基因丰度的影响
DOI：
10.1016/j.jhazmat.2015.06.065
复制
发表时间：
2015
影响因子：
13.6
通讯作者：
Kengara Fredrick Orori
中科院分区：
文献类型：
--
作者：
Sun Mingming;Ye Mao;Wu Jun;Feng Yanfang;Shen Fangyuan;Tian Da;Liu Kuan;Hu Feng;Li Huixin;Jiang Xin;Yang Linzhang;Kengara Fredrick Orori
由于人类活动，土壤暴露于各种类型的化学污染物；然而，对持久性有机污染物和抗生素抗性基因（ARG）的存在的研究有限。据我们所知，目前的工作首次集中于芘和四环素/磺酰胺抗性基因共同污染的土壤的生物修复。孵育 90 天后，芘浓度和四种 ARG（tetW、tetM、sulI 和 sulII）的丰度在不同处理条件下显着降低（p < 0.05）。在结合细菌和槐糖脂处理的微观世界中观察到最大的芘去除率（47.8%）和最大的 ARG 丰度下降（从每 16S rRNA 拷贝 10−7 到 10−8 个 ARG 拷贝）。在整个培养过程中，在接种细菌的微观世界中，芘的生物可及性不断下降。然而，在第 40 天时，在仅用槐糖脂处理的土壤中观察到芘生物可及性和 ARG 丰度增加。 Tenax 提取方法和线性相关分析表明芘的快速解吸分数 (Fr) 和 ARG 丰度之间存在很强的正相关关系。因此，我们得出结论，生物可利用的芘而不是总芘在土壤中 ARG 丰度的维持和波动中起主要作用。
Soils are exposed to various types of chemical contaminants due to anthropogenic activities; however, research on persistent organic pollutants and the existence of antibiotic resistance genes (ARGs) is limited. To our knowledge, the present work for the first time focused on the bioremediation of soil co-contaminated with pyrene and tetracycline/sulfonamide-resistance genes. After 90 days of incubation, the pyrene concentration and the abundance of the four ARGs (tetW,tetM,sulI, andsulII) significantly decreased in different treatment conditions (p< 0.05). The greatest pyrene removal (47.8%) and greatest decrease in ARG abundance (from 10−7to 10−8ARG copies per 16S rRNA copy) were observed in microcosms with a combination of bacterial and sophorolipid treatment. Throughout the incubation, pyrene bioaccessibility constantly declined in the microcosm inoculated with bacteria. However, an increased pyrene bioaccessibility and ARG abundance at day 40 were observed in soil treated with sophorolipid alone. Tenax extraction methods and linear correlation analysis indicated a strong positive relationship between the rapidly desorbing fraction (Fr) of pyrene and ARG abundance. Therefore, we conclude that bioaccessible pyrene rather than total pyrene plays a major role in the maintenance and fluctuation of ARG abundance in the soil.