Antibodies with amylase activity from the sera of autoimmune-prone MRL/MpJ-lpr mice
Antibodies with amylase activity from the sera of autoimmune-prone MRL/MpJ-lpr mice
复制标题
DOI：
10.1016/j.febslet.2006.08.036
复制
发表时间：
2006-09-18
期刊：
影响因子：
3.5
通讯作者：
Nevinsky, Georgy A.
中科院分区：
文献类型：
--
作者：
Andryushkova, Alexandra A.;Kuznetsova, Irina A.;Nevinsky, Georgy A.
自发发生狼疮样自身免疫性病理（SLE）的动物是研究天然抗体酶（Abzs）产生机制及其在自身免疫性疾病病因和发病机制中的作用的非常有希望的模型，但来自动物血清的Abzs几乎尚未被研究。在这项工作中，从MRL/MpJ-lpr小鼠血清中分离出电泳均匀的igg。首次证明了淀粉酶活性是抗体及其分离的重链和轻链的内在特性。SLE病理的各种标志物（蛋白尿、抗dna抗体浓度增强）随着SLE的自发发展而增加，尤其是在动物免疫后，与Abz相对淀粉酶活性的增加相关。健康小鼠血清中淀粉酶活性以分娩后和泌乳初期最高；这与小鼠SLE标志物无关，但支持怀孕可以在健康哺乳动物中“激活”或“触发”自身免疫样表现和Abzs产生的观点。讨论了泌乳小鼠和SLE动物产生Abzs的可能机制差异。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
Animals spontaneously developing lupus-like autoimmune pathology (SLE) are very promising models to study the mechanisms of natural abzymes (Abzs) generation and their role in etiology and pathogenesis of autoimmune diseases, but Abzs from the sera of animals remain virtually unstudied. In this work, electrophoretically homogeneous IgGs were isolated from the sera of MRL/MpJ-lpr mice. It was shown for the first time that amylase activity is an intrinsic property of antibodies (Abs) and their isolated heavy and light chains. Various markers of SLE pathology (proteinuria, enhanced concentration of anti-DNA Abs) increased with spontaneous development of SLE and especially after animal immunization, correlating with the increase in Abz relative amylase activity. The highest amylase activity was found in the sera Abs of healthy mice after delivery and at the beginning of lactation; this was not correlated with markers of mouse SLE but supports the idea that pregnancy could "activate" or "trigger" autoimmune-like manifestations and Abzs production in healthy mammals. The possible differences in mechanisms of Abzs production in lactating mice and animals developing SLE are discussed. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.