Reperfusion damage to the bile canaliculi in transplanted human liver
Reperfusion damage to the bile canaliculi in transplanted human liver
复制标题
移植人肝脏中胆小管的再灌注损伤
DOI：
10.1002/hep.510240512
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发表时间：
1996
期刊：
影响因子：
13.5
通讯作者：
Giuseppe Poli
中科院分区：
文献类型：
--
作者：
J. Cutrin;Dario Cantino;F. Biasi;E. Chiarpotto;Mauro Salizzoni;E. Andorno;Graziella Massano;Giacomo Lanfranco;Mario Rizzetto;Alberto Boveris;Giuseppe Poli
在19例接受原位肝移植（奥尔特）的患者中，根据L-γ-谷氨酰转移酶（GGT）和总胆红素的血浆水平对胆汁淤积的趋势和程度进行了统计学监测。此外，在整个奥尔特过程中检查了胆小管的超微结构，即，在复苏、冷缺血期间和器官再灌注60至90分钟后。胆汁淤积从术后第二天开始明显，在大约10至16天后达到高峰。长期缺血后，在胆小管区域发现肌动蛋白丝功能状态的微小变化。但再灌注后，胆小管的形态发生了显著变化。事实上，小管的平均面积和周长显著增加，单位小管面积的胆汁微绒毛数量明显减少。胆小管似乎是最容易受到缺血再灌注损伤的肝脏结构之一。尤其是在移植肝缺血和复氧后发生的一系列生化变化，将为观察到的早期胆管形态学损伤提供一个原因，这反过来又解释了这些患者在移植后第一个时期的胆汁淤积。
In 19 patients who have undergone orthotopic liver transplantation (OLT), the trend and degree of cholestasis was statistically monitored in terms of plasma levels of L‐gamma‐glutamyl transferase (GGT) and total bilirubin. In addition, the ultrastructure of the bile canaliculus was examined during the entire OLT procedure, i.e., during explantation, cold ischemia, and after 60 to 90 minutes of organ reperfusion. Cholestasis was evident from the second day after surgery, with a peak after approximately 10 to 16 days. Defined, small changes in the functional state of actin filaments were noted in the bile canalicular area after prolonged ischemia. But the morphological status of the bile canaliculi changed dramatically after reperfusion. In fact, the mean area and perimeter of the canaliculi had increased significantly, and there was a marked loss in the number of bile microvilli per unit of canalicular area. The bile canaliculus appears to be one of the liver structures most susceptible to ischemia‐ reperfusion damage. A series of biochemical changes occurring during ischemia and after reoxygenation of the transplanted liver, especially, would provide a reason for the observed early morphological damage of the bile canaliculus, which, in turn, would explain the cholestasis of these patients in the first posttransplantation period.
影响因子：
9
作者：
TZAKIS, A;TODO, S;STARZL, TE
通讯作者：
STARZL, TE
核苷转运蛋白在功能上与大鼠肝小管膜中的核酸外切酶相关。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Che,M;Nishida,T;Gatmaitan,Z;Arias,IM
通讯作者：
Arias,IM
DOI：
--
发表时间：
1989-05
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
P. Phelps;M. Smith;B. Trump
通讯作者：
P. Phelps;M. Smith;B. Trump