Biochemically functionalized silica nanoparticles
Biochemically functionalized silica nanoparticles
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作者：
Qhobosheane, M;Santra, S;Tan, WH
在这份报告中。我们证明了基于二氧化硅的纳米颗粒的生物化学改性。制备了纯的和染料掺杂的二氧化硅纳米颗粒，并用酶和生物相容性化学试剂对其表面进行了修饰，使其能够作为生物传感器和生物标志物。在这项工作中产生的纳米粒子是均匀的大小与1.6%的相对标准偏差。它们具有纯二氧化硅表面，因此可以很容易地用许多生物分子进行修饰，以增加生物化学功能。具体来说，我们已经修改了纳米颗粒表面的酶分子（谷氨酸脱氢酶（GDH）和乳酸脱氢酶（LDH））和生物相容性试剂的细胞膜染色。实验结果表明，二氧化硅纳米粒子是一种生物相容性良好的固定化酶的固体载体。纳米颗粒表面的固定化酶分子在其各自的酶促反应中显示出优异的酶促活性。纳米颗粒表面生化功能化证明了使用纳米颗粒用于生物传感和生物识别应用的可行性。
In this report. we demonstrate the biochemical modification of silica based nanoparticles. Both pure and dye-doped silica nanoparticles were prepared, and their surfaces were modified with enzymes and biocompatible chemical reagents that allow them to function as biosensors and biomarkers. The nanoparticles produced in this work are uniform in size with a 1.6% relative standard deviation. They have a pure silica surface and can thus be modified easily with many biomolecules for added biochemical functionality. Specifically, we have modified the nanoparticle surfaces with enzyme molecules (glutamate dehydrogenase (GDH) and lactate dehydrogenase (LDH)) and a biocompatible reagent for cell membrane staining. Experimental results show that the silica nanoparticles are a good biocompatible solid support for enzyme immobilization. The immobilized enzyme molecules on the nanoparticle surface have shown excellent enzymatic activity in their respective enzymatic reactions. The nanoparticle surface biochemical functionalization demonstrates the feasibility of using nanoparticles for biosensing and biomarking applications.