SUPPRESSION OF A VP1 MUTANT OF SIMIAN VIRUS-40 BY MISSENSE MUTATIONS IN SERINE CODONS OF THE VIRAL AGNOGENE
SUPPRESSION OF A VP1 MUTANT OF SIMIAN VIRUS-40 BY MISSENSE MUTATIONS IN SERINE CODONS OF THE VIRAL AGNOGENE
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DOI：
10.1128/jvi.48.2.405-409.1983
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
NATHANS, D
中科院分区：
文献类型：
--
作者：
MARGOLSKEE, RF;NATHANS, D
分离出SV40部分缺陷型VP1突变体的第二位点回复突变体。这些假回复突变体中的每一个中的抑制突变被定位到病毒的未知基因。6个独立分离的假回复突变体，都有一个错义突变的丝氨酸密码子，附近的开始的未知基因，这将导致取代丝氨酸在位置7，11，或17的未知蛋白的疏水氨基酸。显然，在病毒发育的后期阶段，该未知蛋白以特定的方式与VP1相互作用。
Second-site revertants of a partially defective VP1 mutant of SV40 were isolated. The suppressing mutation in each of these pseudorevertants was mapped to the viral agnogene. Of 6 independently isolated pseudorevertants, all had a missense mutation in a serine codon, near the beginning of the agnogene, that would cause replacement of serine at position 7, 11, or 17 in the agnoprotein by a hydrophobic amino acid. Evidently, the agnoprotein interacts in a specific way with VP1 during the late stages of viral development.