Developmental expression and perturbation of arabinogalactan-proteins during seed germination and seedling growth in tomato
Developmental expression and perturbation of arabinogalactan-proteins during seed germination and seedling growth in tomato
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DOI：
10.1034/j.1399-3054.2001.1120319.x
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发表时间：
2001-07-01
影响因子：
6.4
通讯作者：
Showalter, AM
中科院分区：
文献类型：
--
作者：
Lu, H;Chen, M;Showalter, AM
阿拉伯半乳聚糖蛋白 (AGP) 是一类高度糖基化、富含羟脯氨酸的糖蛋白，存在于整个植物界。 (β-D-Gal)(3) Yariv 试剂是一种合成化学试剂，可特异性结合 AGP，可用于组织化学染色、分离和探测其功能。 AGP 的数量。在这里，AGP 在番茄 (Lycopersicon esulentum Mill. cv. UC82B) 种子萌发和幼苗生长中的作用通过跟踪这些事件期间 AGP 的表达以及用 (β-D-Gal)(3) Yariv 处理扰乱 AGP 功能来检查，AGP 表达在萌发和幼苗发育过程中定量和定性地发生变化，如总 AGP 含量、交叉电泳图谱、RNA 印迹分析所示。使用 LeAGP-1 探针，以及使用 LeAGP-1、JIM13 和 MAC207 抗体进行蛋白质印迹。 (β-D-Gal)(3) 对种子和发育中的幼苗进行 Yariv 处理不会影响种子发芽率，但会显着抑制根部幼苗的生长，并在较小程度上抑制芽的生长。根生长抑制包括总根长度、表皮根细胞伸长、根细胞数量和根毛形成的减少。在去除(β-D-Gal)(3) Yariv后，这种生长抑制是可逆的。在相关实验中，(β-D-Gal)(3) Yariv处理极大地抑制了番茄幼苗的水分吸收。基于这些实验，AGP 明显与番茄幼苗发育相关，并且可能在根生长中发挥作用，更具体地说，在细胞伸长、细胞增殖、根毛形成和水分吸收中发挥作用。
Arabinogalactan-proteins (AGPs) are a family of highly glycosylated hydroxyproline-rich glycoproteins present throughout the plant kingdom. A synthetic chemical reagent, (beta -D-Gal)(3) Yariv reagent, specifically binds AGPs and can be used for histochemical staining, isolating and probing the function. of AGPs. Here, the role of AGPs in tomato (Lycopersicon esulentum Mill. cv. UC82B) seed germination and seedling growth was examined by following expression of AGPs during these events and by treatment with (beta -D-Gal)(3) Yariv to perturb AGP function, AGP expression changed during germination and seedling development both quantitatively and qualitatively as revealed by analysis of total AGP content, crossed electrophoresis patterns, RNA blots using LeAGP-1 probe, and western blots with LeAGP-1, JIM13, and MAC207 antibodies. (beta -D-Gal)(3) Yariv treatment of seeds and developing seedlings did not affect percent seed germination, but markedly inhibited seedling growth in roots and to a lesser degree in shoots. Root growth inhibition encompassed reductions in overall root length, epidermal root cell elongation, root cell numbers and root hair formation. This growth inhibition was reversible following removal of (beta -D-Gal)(3) Yariv, In a related experiment, water uptake by tomato seedlings was greatly inhibited by (beta -D-Gal)(3) Yariv treatment. Based on these experiments, AGPs are clearly associated with tomato seedling development and likely to function in root growth, more specifically in cell elongation, cell proliferation, root hair formation and water uptake.