Electrochemical estrogen screen method based on the electrochemical behavior of MCF-7 cells
Electrochemical estrogen screen method based on the electrochemical behavior of MCF-7 cells
复制标题
基于MCF-7细胞电化学行为的电化学雌激素筛选方法
DOI：
10.1016/j.jhazmat.2015.09.031
复制
发表时间：
2016
影响因子：
13.6
通讯作者：
Dongmei Wu
中科院分区：
文献类型：
--
作者：
Jinlian Li;Jia Song;Sheng Bi;Shi Zhou;Jiwen Cui;Jiguang Liu;Dongmei Wu
因此，建立快速、廉价、准确的雌激素筛选方法来评价新兴化学品的雌激素效应是一项迫切的任务。本研究比较了不含雌激素和正常破碎MCF-7细胞悬液的伏安行为，发现不含雌激素的破碎MCF-7细胞悬液的电化学信号（约0.68 V，由黄嘌呤和鸟嘌呤引起）明显低于正常细胞悬液。外分泌嘌呤的电化学检测表明，由于内源性雌激素的清除，细胞外分泌嘌呤的能力急剧下降。结果表明，MCF-7细胞的电化学信号与细胞内雌激素水平有关。当细胞内雌激素水平下调时，黄嘌呤和次黄嘌呤的浓度降低，导致MCF-7细胞的电化学信号下降。基于电化学信号，建立了电化学雌激素筛选方法。用电化学方法评价了雌二醇、壬基酚和双酚A的雌激素效应，其结果与MTT法一致。电化学雌激素筛选方法具有简便、快速、廉价、客观等优点，为评价化学物质的雌激素效应开辟了一条新的途径。
It was an urgent task to develop quick, cheap and accurate estrogen screen method for evaluating the estrogen effect of the booming chemicals. In this study, the voltammetric behavior between the estrogen-free and normal fragmented MCF-7 cell suspensions were compared, and the electrochemical signal (about 0.68 V attributed by xanthine and guanine) of the estrogen-free fragmented MCF-7 cell suspension was obviously lower than that of the normal one. The electrochemistry detection of ex-secretion purines showed that the ability of ex-secretion purines of cells sharp decreased due to the removing of endogenous estrogen. The results indicated that the electrochemical signal of MCF-7 cells was related to the level of intracellular estrogen. When the level of intracellular estrogen was down-regulated, the concentrations of the xanthine and hypoxanthine decreased, which led to the electrochemical signal of MCF-7 cells fall. Based on the electrochemical signal, the electrochemical estrogen screen method was established. The estrogen effect of estradiol, nonylphenol and bisphenol A was evaluated with the electrochemical method, and the result was accordant with that of MTT assay. The electrochemical estrogen screen method was simple, quickly, cheap, objective, and it exploits a new way for the evaluation of estrogenic effects of chemicals.