The Gastric Microbiome Is Perturbed in Advanced Gastric Adenocarcinoma Identified Through Shotgun Metagenomics
The Gastric Microbiome Is Perturbed in Advanced Gastric Adenocarcinoma Identified Through Shotgun Metagenomics
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DOI：
10.3389/fcimb.2018.00433
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发表时间：
2018-12-12
影响因子：
5.7
通讯作者：
Zhang, Chao-Jun
中科院分区：
文献类型：
--
作者：
Hu, Yuan-Liang;Pang, Wei;Zhang, Chao-Jun
目的：幽门螺杆菌等胃微生物群失调在胃癌的发病机制和进展中发挥重要作用。我们的目的是评估浅表性胃炎（SG）和晚期胃腺癌（GC）中胃微生物群的组成和功能影响。方法：我们对 6 例 GC 患者和 5 例 SG 患者的胃洗液样本进行鸟枪法宏基因组测序。使用 MetaPhlAn2 分析分类组成，使用 HUMAnN2 分析功能基因途径。通过 LEfSe 评估两个患者组之间微生物组成和途径的差异。结果：GC 患者胃微生物群的特点是物种丰富度降低、13 种细菌类群富集和 31 种细菌类群缺失 (q < 0.05)。 GC中丰富的最具代表性的分类单元对应于通常定植于口腔的共生菌或机会致病菌，包括奈瑟菌属、异普氏菌属和聚合杆菌属、肺炎链球菌属和牙髓卟啉单胞菌属。 t_GCF_000174815。三个GC相关属中的每一个都可以将GC与SG完全分开。特别是，Sphingobium yanoikuyae（一种能够降解致癌化合物的细菌）在 GC 中被耗尽。从功能上来说，与脂多糖（LPS）和L-精氨酸生物合成相关的通路在GC中更丰富，而涉及短链脂肪酸（SCFA）发酵和支链氨基酸代谢的通路在SG中更丰富。结论：我们的结果从全基因组的角度呈现了GC患者胃微生物组的新变化，表明微生物组的组成和功能可用于GC的预后和诊断。
Objective: Dysbiosis of gastric microbiota such as Helicobacter pylori plays a significant role in pathogenesis and progression of gastric cancer. Our aim was to evaluate the composition and functional effects of gastric microbiota in superficial gastritis (SG) and advanced gastric adenocarcinoma (GC).Methods: We carried out shotgun metagenomic sequencing on gastric wash samples from 6 patients with GC and 5 patients with SG. The taxonomic composition was profiled using MetaPhlAn2 and functional gene pathway was profiled using HUMAnN2. Differences in microbial composition and pathways between the two patient groups were assessed via LEfSe.Results: The gastric microbiota in GC patients was characterized by reduced species richness, enrichment of 13 bacterial taxa and depletion of 31 taxa (q < 0.05). The most representative taxa which were abundant in GC corresponded to the commensals or opportunistic pathogens that usually colonize the oral cavity, including genera Neisseria, Alloprevotella, and Aggregatibacter, species Streptococcus_mitis_oralis_ pneumoniae and strain Porphyromonas_endodontalis. t_GCF_000174815. Each of the three GC-associated genera could separate GC from SG completely. In particular, Sphingobium yanoikuyae, a bacterium capable of degrading carcinogenic compounds, was depleted in GC. Functionally, pathways associated with the biosynthesis of lipopolysaccharide (LPS) and L-arginine were enriched in GC, whereas pathways involved in the fermentation of short chain fatty acids (SCFAs) and branched amino acid metabolism were more abundant in SG.Conclusions: Our results present new alterations in the gastric microbiome in patients with GC from a whole-genome perspective, suggesting that microbiome composition and function can be used for prognosis and diagnosis of GC.