Improvement of welan gum production and redistribution of metabolic flux under pH control process in Alcaligenes sp CGMCC2428
Improvement of welan gum production and redistribution of metabolic flux under pH control process in Alcaligenes sp CGMCC2428
复制标题
产碱菌 CGMCC2428 pH 控制过程中韦兰胶生产的改进和代谢通量的重新分配
DOI：
10.1007/s12257-012-0423-5
复制
发表时间：
2013-04-01
影响因子：
3.2
通讯作者：
Ouyang, Pingkai
中科院分区：
文献类型：
--
作者：
Li, Hui;Li, Sha;Ouyang, Pingkai
Batch fermentations of welan gum from Alcaligenes sp. CGMCC2428 at pH values of 5.5 similar to 7.0 were studied. Based on the kinetic analysis, a pH control process for improving welan production was developed. By maintaining the culture pH at 7.0, the process significantly improved the maximal welan concentration and productivity to reach 25.1 +/- 0.65 g/L and 0.42 +/- 0.003 g/L/h, respectively, compared with those in native pH processes where pH value would decrease from 7.0 to 5.1 (18.5 +/- 0.72 g/L and 0.28 +/- 0.002 g/L/h). This improvement may be due to the increased metabolic flux of glucose-1-phosphate to welan induced by pH control process. Furthermore, scale-up fermentation under controlled pH was implemented using 300-L fermentor. The highest welan concentration of 27.5 +/- 0.97 g/L was obtained. This simplified process proved effective in industry fermentation for high welan production.
