Analysis of the Immune and Antioxidant Response of Cellulose Nanocrystals Grafted with β -Cyclodextrin in Myeloid Cell Lines
Analysis of the Immune and Antioxidant Response of Cellulose Nanocrystals Grafted with β -Cyclodextrin in Myeloid Cell Lines
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骨髓细胞系中接枝β-环糊精的纤维素纳米晶体的免疫和抗氧化反应分析
DOI：
10.1155/2019/4751827
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发表时间：
2019
影响因子：
--
通讯作者：
Ckless, Karina
中科院分区：
文献类型：
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作者：
Sunasee, Rajesh;Carson, Melissa;Despres, Hannah W.;Pacherille, Angela;Nunez, Kaylee D.;Ckless, Karina
纤维素纳米晶(CNCs)在许多领域的研究、应用和未来的商业化前景方面都具有巨大的潜力。最近，碳纳米管已成为药物和基因传递系统等生物医学应用的有吸引力的候选材料。因此，细胞毒性研究一直是过去十年的主要焦点。然而，尽管碳纳米管具有棒状的性质，但表面修饰的碳纳米管的潜在免疫应答还没有得到很好的研究。本研究在人单核细胞系(β-1)和小鼠巨噬细胞样细胞系(J774A.1)上检测了CNCS与环糊精(CNCS-β-CD)复合后可能诱导的免疫和抗氧化反应。用ELISA法分析促炎症细胞因子白介素1β(IL-1β)的分泌，用荧光细胞成像法检测线粒体衍生的活性氧(ROS)，用免疫印迹法检测细胞内参与免疫和抗氧化反应的蛋白水平。我们的结果表明，在不同浓度的CNCS-β-CD处理的THP-1细胞中，IL-1β的分泌和线粒体来源的ROS都没有显着增加，导致细胞内的抗氧化反应没有改变。总体而言，CNCS-β-CD是非免疫原性的，在所测试的条件下不会诱导增强的抗氧化反应，因此有可能被用作药物输送载体。
Cellulose nanocrystals (CNCs) have great potential in many areas of research, applications, and future commercialization prospects. Recently, CNCs have emerged as attractive candidates for biomedical applications such as drug and gene delivery systems. As such, cytotoxicity studies have been the major focus in the past decade. However, despite the rod‐like nature of CNCs, the potential immune response of surface‐modified CNCs is not well investigated. The current study examined the potential immune and antioxidant response induced by CNCs grafted withβ‐cyclodextrin (CNCs‐β‐CD) in a human monocyte cell line (THP‐1) and a mouse macrophage‐like cell line (J774A.1). We analyzed the secretion of the proinflammatory cytokine, interleukin 1β(IL‐1β), by ELISA and mitochondria‐derived reactive oxygen species (ROS) using fluorescence cell imaging and examined the intracellular levels of proteins involved in the immune and antioxidant response by immunoblotting. Our results indicated a dramatic increase neither in the IL‐1βsecretion nor in the mitochondria‐derived ROS resulting in no changes in the intracellular antioxidant response in THP‐1 cells treated with different concentrations of CNCs‐β‐CD. Overall, CNCs‐β‐CD is nonimmunogenic and do not induce an increased antioxidant response under the conditions tested and hence has the potential to be used as a drug delivery carrier.