Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies
Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies
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DOI：
10.1007/s00216-006-0470-x
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发表时间：
2006-05
影响因子：
4.3
通讯作者：
A. Yamaguchi;M. Nakano;Kimihisa Nochi;Tomohisa Yamashita;Kotaro Morita;N. Teramae
中科院分区：
文献类型：
--
作者：
A. Yamaguchi;M. Nakano;Kimihisa Nochi;Tomohisa Yamashita;Kotaro Morita;N. Teramae
通过二次谐波发生（SHG）和荧光光谱研究了一系列半花青染料对磷脂囊泡膜的吸附和纵向扩散行为。据观察，阳离子半花青染料的纵向扩散在这些染料最初吸附到囊泡膜的外表面之后立即发生。相比之下，在测量时间内（<2000 s）几乎没有任何量的具有磺酸盐基团的两性离子半花青染料扩散穿过囊泡膜。基于所有测试的半花青染料的倍频和荧光光谱的时程响应的差异，我们提出磺酸基的水合是两性离子半花青染料的低扩散率的主要原因。
The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.