Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment
Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment
复制标题
DOI：
10.1111/j.1399-302x.2009.00542.x
复制
发表时间：
2009-12-01
影响因子：
--
通讯作者：
Grenier, D.
中科院分区：
文献类型：
--
作者：
Grenier, G.;Gagnon, G.;Grenier, D.
背景/目的：尽管细菌在牙周炎病因学中的作用已被充分确定，但有人认为疱疹病毒可能导致这种疾病的发生和进展。本研究的目的是确定从牙周健康患者、牙龈炎和牙周炎患者获得的龈沟液（GCF）样本中人类巨细胞病毒（HCMV）、EB病毒（EBV）和单纯疱疹病毒（HSV）的流行情况。此外，在患有慢性牙周炎的患者亚组中评估了牙周治疗（洁治和根面平整）对疱疹病毒持久性的影响。方法：通过巢式 PCR 扩增技术评估 GCF 样本中病毒的存在。在洗牙和根面平整治疗后评估牙周部位病毒的持续性。结果：与健康对照受试者相比，牙周炎患者中观察到的 HCMV 患病率具有统计学意义（分别为 35% 和 8%；P = 0.0377）。与健康对照受试者相比，牙周炎组的 HSV 患病率也呈较高趋势。此外，HCMV 的较高患病率与患有牙周炎的受试者的深牙周袋有关。在牙周炎患者亚组中，牙周治疗导致疱疹病毒的消除（HCMV和EBV）或减少（HSV）。结论：本研究表明，与牙周健康受试者相比，牙周炎患者GCF中HCMV和HSV病毒的患病率较高，并且深牙周袋中HCMV的患病率较高。它还提供了证据表明牙周治疗可能与清除患病部位的病毒有关。
Background/Aim:Although the role of bacteria in the etiology of periodontitis is well established, it has been suggested that herpetic viruses could contribute to the initiation and progression of this disease. The aim of this study was to determine the prevalence of human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and herpes simplex virus (HSV) in gingival crevicular fluid (GCF) samples obtained from periodontally healthy, gingivitis and periodontitis patients. In addition, the effect of periodontal treatment (scaling and root planing) on the persistence of herpetic viruses was evaluated in a sub-group of patients suffering from chronic periodontitis.Methods:The presence of viruses in GCF samples was assessed by a nested PCR amplification technique. The persistence of viruses in periodontal sites was evaluated following a scaling and root planing therapy.Results:A statistically significant higher prevalence of HCMV was observed in periodontitis patients as compared to healthy control subjects (35 vs. 8%, respectively; P = 0.0377). A trend for a higher prevalence of HSV was also noted in the periodontitis group, in comparison with healthy control subjects. In addition, a higher prevalence of HCMV was associated with deep periodontal pockets in subjects suffering from periodontitis. In the sub-group of periodontitis patients, periodontal therapy resulted in the elimination (HCMV and EBV) or reduction (HSV) of the herpetic viruses.Conclusions:This study showed that the prevalence of HCMV and HSV viruses in GCF is higher in patients suffering from periodontitis compared to periodontally healthy subjects, and that the prevalence of HCMV is higher in deep periodontal pockets. It also brought evidences that periodontal therapy may be associated with virus elimination in diseased sites.