Spatial and temporal patterns of cell death in limb bud mesoderm after apical ectodermal ridge removal.
Spatial and temporal patterns of cell death in limb bud mesoderm after apical ectodermal ridge removal.
复制标题
顶端外胚层脊去除后肢芽中胚层细胞死亡的空间和时间模式。
DOI：
10.1016/0012-1606(82)90241-x
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
Fallon,JF
中科院分区：
文献类型：
--
作者：
Rowe,DA;Cairns,JM;Fallon,JF
去除顶端外胚脊后，雏鸡翅膀和腿芽中胚层出现了细胞死亡的时空模式。细胞死亡的区域从肢芽远端表面延伸到150-200μm进入中胚层。在发育后期去掉脊状突起的肢芽没有表现出细胞死亡的时空模式。在去除背侧外胚层的对照实验中，没有出现细胞死亡的模式。去除脊状突起和预期细胞死亡的150-200μm区域的一部分，导致细胞死亡的区域大约等于剩余区域的数量。在去除所有预期的细胞死亡区和根尖脊后，在剩下的肢芽中胚层观察到细胞死亡。在这些肢芽中，细胞死亡发生在肢芽中未见的区域，仅去除了顶端的脊线。我们的结论是，在第18-20期，脊下150-200μm的中胚层细胞需要顶脊存活。更多的近端中胚层细胞不会在仅去除脊线后死亡，但显然需要更远端的中胚层的存在才能存活。这是否是对远端中胚层所固有的东西的要求，还是它通过脊线所拥有的东西，尚不清楚。23期之后，去除根尖脊后，肢体中胚层细胞不会死亡。然而，在后期阶段，翼芽近端-远端伸长和远端肢体成分的分化仍然需要脊状突起。
A spatiotemporal pattern of cell death occurred in the chick wing and leg bud mesoderm after removal of apical ectodermal ridge at stages 18–20. Cells died in a region extending from the limb bud distal surface to 150–200 μm into the mesoderm. Limb buds from which ridge was removed at later stages in development did not exhibit a spatiotemporal pattern of cell death. In control experiments in which dorsal ectoderm was removed, a pattern of cell death did not occur. Removal of the ridge and part of the 150- to 200-μm zone of prospective cell death resulted in cell death in an area approximately equal to the amount of the zone remaining. After removal of all of the prospective zone of cell death plus the apical ridge, cell death was observed in the remaining limb bud mesoderm. In these limb buds, cell death occurred in a region in which it had not been seen in limb bud with apical ridge alone removed. We conclude that at stages 18–20 the mesodermal cells 150–200 μm beneath the ridge require the apical ridge to survive. More proximal mesodermal cells do not die after ridge removal alone, but apparently require the presence of the more distal mesoderm to survive. Whether this is a requirement for something intrinsic to the distal mesoderm or something it possesses by way of the ridge is unknown. After stage 23, the limb mesoderm cells do not die when the apical ridge is removed. Nevertheless, at the later stages, ridge continues to be required for limb bud proximal-distal elongation and the differentiation of distal limb elements.