NASA-Approved Rotary Bioreactor Enhances Proliferation and Osteogenesis of Human Periodontal Ligament Stem Cells
NASA-Approved Rotary Bioreactor Enhances Proliferation and Osteogenesis of Human Periodontal Ligament Stem Cells
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DOI：
10.1089/scd.2008.0371
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发表时间：
2009-11-01
影响因子：
4
通讯作者：
Ni, Longxing
中科院分区：
文献类型：
--
作者：
Li, Shi;Ma, Zhaofeng;Ni, Longxing
研究表明牙周膜干细胞（PDLSCs）在牙周组织缺损的修复中起着重要作用，近年来基于PDLSCs的组织工程牙周组织再生成为牙周组织研究的热点。实现这一目标的理论方法是提供一种“刺激”环境，以在体外快速扩增PDLSC，从而加速牙周组织工程。我们推测三维动态模拟微重力培养系统对牙周干细胞有影响，有利于牙周干细胞的增殖和分化，但目前尚无相关报道。本研究探讨旋转细胞培养系统（RCCS）诱导的三维动态SMG对人牙周膜干细胞（hPDLSCs）的生物学效应。从手术提取的人牙齿中分离hPDLSC，并通过收集多个集落进行富集。将hPDLSC接种在Cytodex 3微载体上并在RCCS中培养。结果表明，SMG对hPDLSC的生物学行为有影响，表现为促进hPDLSC的增殖和活力、改变形态、破坏微丝系统。此外，SMG处理的hPDLSCs在成骨培养基中孵育后表现出基质矿化增加和矿化相关基因的表达上调。由于这是首次研究SMG对PDLSC的影响，该研究可能有助于了解3D环境中细胞反应的变化，并有助于利用基于PDLSC的组织工程方法实现理想的牙周再生。
Previous studies have suggested that periodontal ligament stem cells (PDLSCs) play crucial role in regeneration of periodontal defects, and recently tissue engineering based on PDLSCs to enhance periodontal regeneration has been the focus of periodontal research. A theoretical way to achieve this goal would be to provide a "stimulatory'' environment to rapidly expand PDLSCs in vitro to expedite tissue engineering of periodontium. We hypothesize that three-dimensional (3D) dynamic simulated microgravity (SMG) culture system have effect on periodontal stem cells, and would benefit periodontal stem cells proliferation and differentiation, but up to now, there are no related reports on this aspect. In this study, we investigated the biological effect of three-dimensional dynamic SMG induced by rotary cell culture system (RCCS) on human periodontal ligament stem cells (hPDLSCs) in vitro. hPDLSCs were isolated from surgically extracted human teeth and enriched by collecting multiple colonies. hPDLSCs were inoculated on Cytodex 3 microcarriers and cultured in RCCS. The results showed that SMG affected the biology of hPDLSCs as indicated by promotion of proliferation and viability, alterations of morphology, and disorganization of microfilament system. Besides, SMG-treated hPDLSCs presented increased matrix mineralization and up-regulated expression of mineralization associated genes after incubation in osteogenic medium. For it is the first time to investigate effects of SMG on PDLSCs, the research may lend insight into variations of cell response in 3D environment, and contribute to achievement of desirable periodontal regeneration utilizing PDLSCs-based tissue engineering approaches.