CRISPR/Cas Technologies and Their Applications in Escherichia coli.
CRISPR/Cas Technologies and Their Applications in Escherichia coli.
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DOI：
10.3389/fbioe.2021.762676
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发表时间：
2021
影响因子：
5.7
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Dong H;Cui Y;Zhang D
成簇的规则间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）系统彻底改变了基因组编辑，极大地促进了生物技术的发展。然而，不幸的是，这些系统还没有被开发和应用在细菌中广泛的真核生物。本文就目前应用最广泛的CRISPR/Cas工具及其在大肠杆菌中的应用研究进展进行综述。基因组编辑的基础上同源重组，非同源DNA末端连接，转座子和碱基编辑器进行了讨论。最后，简要回顾了使用CRISPRi进行转录调控的最新进展。该综述为CRISPR/Cas系统在其他细菌物种中的应用提供了有益的参考。
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.