Resistance of the constitutive microflora of biofilms formed on whey reverse-osmosis membranes to individual cleaning steps of a typical clean-in-place protocol
Resistance of the constitutive microflora of biofilms formed on whey reverse-osmosis membranes to individual cleaning steps of a typical clean-in-place protocol
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DOI：
10.3168/jds.2013-7012
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发表时间：
2013-10-01
影响因子：
3.5
通讯作者：
Singh, Diwakar
中科院分区：
文献类型：
--
作者：
Anand, Sanjeev;Singh, Diwakar
该实验评估了在工业加工条件下，就地清洁方案的各个步骤对从乳清反渗透膜上形成的生物膜中分离的生物膜组成性微生物群落的有效性，所述乳清反渗透膜老化2至14个月。用于体外耐药性研究的分离株包括芽孢杆菌属、肠球菌属、链球菌属、葡萄球菌属、微球菌属、气单胞菌属、棒状杆菌属、假单胞菌属、克雷伯菌属和埃希氏菌属。6个清洁步骤（碱、表面活性剂、酸、酶、第二种表面活性剂和消毒剂处理）显示分离株在渗透压和生物膜包埋细胞状态下均具有抗性。最有效的步骤是酸处理，这导致在4.54至7.90和2.09至5.02对数减少的渗透压和生物膜包埋的细胞，分别。尽管消毒剂步骤在嗜酸性细胞的情况下导致4.91至8.33 log的减少，但其对生物膜包埋的细胞的有效性较低，导致0.59至1.64 log的减少。芽孢杆菌显示出最高的电阻，在两个反渗透，以及嵌入式单元状态。
This experiment evaluates the effectiveness of individual steps of a clean-in-place protocol against the biofilm constitutive microflora isolated from the biofilms developed on whey reverse-osmosis membranes, aged 2 to 14 mo, under industrial processing conditions. The isolates used for the in vitro resistance studies included species of Bacillus, Enterococcus, Streptococcus, Staphylococcus, Micrococcus, Aeromonas, Corynebacterium, Pseudomonas, Klebsiella, and Escherichia. The 6 cleaning steps (alkali, surfactant, acid, enzyme, a second surfactant, and sanitizer treatment) revealed resistance of isolates in both planktonic and biofilm-embedded cell states. The most effective step was the acid treatment, which resulted in 4.54 to 7.90 and 2.09 to 5.02 log reductions of the planktonic and biofilm-embedded cells, respectively. Although the sanitizer step causing a reduction of 4.91 to 8.33 log in the case of planktonic cells, it was less effective against the biofilm-embedded cells, resulting in a reduction of 0.59 to 1.64 log. Bacillus spp. showed the highest resistance in both planktonic, as well as embedded cell states.