emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex.
emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex.
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DOI：
10.1534/genetics.111.131714
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发表时间：
2011-10
期刊：
影响因子：
3.3
通讯作者：
Golden A
中科院分区：
文献类型：
--
作者：
Shakes DC;Allen AK;Albert KM;Golden A
在线虫秀丽隐杆线虫中，emb-1的温度敏感突变体以与已经耗尽后期促进复合物或细胞体（APC/C）的已知亚基的胚胎难以区分的方式在减数分裂中期I停滞为单细胞胚胎。在这里，我们表明，emb-1表型增强与已知的APC/C亚基的双突变体组合和抑制与已知的APC/C抑制剂的双突变体组合。除了其减数分裂功能，emb-1是生殖系有丝分裂增殖所必需的。这些研究表明，emb-1编码K10D2.4，这是最近发现的小APC/C亚基APC 16的同源物。
In the nematode Caenorhabditis elegans, temperature-sensitive mutants of emb-1 arrest as one-cell embryos in metaphase of meiosis I in a manner that is indistinguishable from embryos that have been depleted of known subunits of the anaphase-promoting complex or cyclosome (APC/C). Here we show that the emb-1 phenotype is enhanced in double mutant combinations with known APC/C subunits and suppressed in double mutant combinations with known APC/C suppressors. In addition to its meiotic function, emb-1 is required for mitotic proliferation of the germline. These studies reveal that emb-1 encodes K10D2.4, a homolog of the small, recently discovered APC/C subunit, APC16.