Analysis of Replication Intermediates Indicates That Drosophila melanogaster Mitochondrial DNA Replicates by a Strand-Coupled Theta Mechanism
Analysis of Replication Intermediates Indicates That Drosophila melanogaster Mitochondrial DNA Replicates by a Strand-Coupled Theta Mechanism
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DOI：
10.1371/journal.pone.0053249
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发表时间：
2013-01-04
期刊：
影响因子：
3.7
通讯作者：
Jacobs, Howard T.
中科院分区：
文献类型：
--
作者：
Joers, Priit;Jacobs, Howard T.
线粒体DNA合成对于细胞器的正常功能和整个真核生物体是必需的。在这里，我们证明，使用二维琼脂糖凝胶电泳分析复制中间体，单向，链偶联的DNA合成是普遍的模式mtDNA复制在果蝇。在单个延伸的非编码区（NCR）内开始，复制围绕环状基因组进行，表现出不规则的延伸速率，并在特定区域频繁暂停。在mtDNA分子的一个子集中发现了链异步DNA合成的有限贡献的证据，但仅限于NCR下游的核糖体RNA基因区域。我们的研究结果表明，链偶联复制是广泛的后生动物，并应告知未来的研究线粒体DNA代谢的D。黑腹果蝇
Mitochondrial DNA synthesis is necessary for the normal function of the organelle and for the eukaryotic organism as a whole. Here we demonstrate, using two-dimensional agarose gel electrophoresis to analyse replication intermediates, that unidirectional, strand-coupled DNA synthesis is the prevalent mode of mtDNA replication in Drosophila melanogaster. Commencing within the single, extended non-coding region (NCR), replication proceeds around the circular genome, manifesting an irregular rate of elongation, and pausing frequently in specific regions. Evidence for a limited contribution of strand-asynchronous DNA synthesis was found in a subset of mtDNA molecules, but confined to the ribosomal RNA gene region, just downstream of the NCR. Our findings imply that strand-coupled replication is widespread amongst metazoans, and should inform future research on mtDNA metabolism in D. melanogaster.