Engineered Chimeric Peptides as Antimicrobial Surface Coating Agents toward Infection-Free Implants.
Engineered Chimeric Peptides as Antimicrobial Surface Coating Agents toward Infection-Free Implants.
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DOI：
10.1021/acsami.5b03697
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发表时间：
2016-03-02
影响因子：
9.5
通讯作者：
Tamerler C
中科院分区：
文献类型：
--
作者：
Yazici H;O'Neill MB;Kacar T;Wilson BR;Oren EE;Sarikaya M;Tamerler C
预防细菌定植和随之而来的生物膜形成仍然是植入式医疗器械的主要挑战。种植体相关感染不仅是种植体失败的主要原因，而且由于对多种细菌的多药耐药性的升级，常规抗生素治疗会带来进一步的并发症。由于其独特的特性，抗菌肽（AMPs）作为对抗微生物定植的有效药物受到了极大的关注。这些肽已被证明对靶细胞具有广泛的特异性活性，同时对细菌产生耐药性的倾向较低。因此，具有AMP特性的工程生物材料表面为预防种植体感染提供了一种很有前途的方法。在这里，我们设计了一种具有双功能的嵌合肽，在呈现抗菌性能的同时形成坚固的固体表面涂层。嵌合肽的各个结构域被评价了它们与钛底物的固体结合动力学以及它们在溶液中的抗菌性能。体外实验评价了嵌合肽对多种细菌（包括变形链球菌、葡萄球菌）感染的抑菌效果。以及大肠杆菌，这些细菌常见于口腔和骨科种植体相关手术中。我们的结果表明，在减少细菌定植到钛表面低于可检测限度显著改善。具有自由显示抗菌结构域的工程嵌合肽可能是通过高度减少细菌定植特性的工程植入界面开发无感染表面的潜在解决方案。
Prevention of bacterial colonization and consequent biofilm formation remains a major challenge in implantable medical devices. Implant-associated infections are not only a major cause of implant failures but also their conventional treatment with antibiotics brings further complications due to the escalation in multidrug resistance to a variety of bacterial species. Owing to their unique properties, antimicrobial peptides (AMPs) have gained significant attention as effective agents to combat colonization of microorganisms. These peptides have been shown to exhibit a wide spectrum of activities with specificity to a target cell while having a low tendency for developing bacterial resistance. Engineering biomaterial surfaces that feature AMP properties, therefore, offer a promising approach to prevent implant infections. Here, we engineered a chimeric peptide with bifunctionality that both forms a robust solid-surface coating while presenting antimicrobial property. The individual domains of the chimeric peptides were evaluated for their solid-binding kinetics to titanium substrate as well as for their antimicrobial properties in solution. The antimicrobial efficacy of the chimeric peptide on the implant material was evaluated in vitro against infection by a variety of bacteria, including Streptococcus mutans, Staphylococcus. epidermidis, and Escherichia coli, which are commonly found in oral and orthopedic implant related surgeries. Our results demonstrate significant improvement in reducing bacterial colonization onto titanium surfaces below the detectable limit. Engineered chimeric peptides with freely displayed antimicrobial domains could be a potential solution for developing infection-free surfaces by engineering implant interfaces with highly reduced bacterial colonization property.