Characterization ofColletotrichum ocimiPopulation Associated with Black Spot of Sweet Basil (Ocimum basilicum) in Northern Italy
Characterization ofColletotrichum ocimiPopulation Associated with Black Spot of Sweet Basil (Ocimum basilicum) in Northern Italy
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DOI：
10.3390/plants9050654
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发表时间：
2020-05-01
期刊：
影响因子：
4.5
通讯作者：
Gullino, Maria Lodovica
中科院分区：
文献类型：
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作者：
Cacciola, Santa Olga;Gilardi, Giovanna;Gullino, Maria Lodovica
黑斑病是甜罗勒（Ocimum basilicum）的主要叶部病害，存在于北方意大利的典型栽培区，包括利古里亚和南皮埃蒙特地区，在那里这种芳香草本植物是经济上重要的作物。本研究对从该地区甜罗勒植株上采集的15个炭疽菌菌株进行了形态学和核DNA分析，并利用内转录间隔区（ITS）、5.8S nrDNA和部分β-微管蛋白基因（TUB 2）作为条形码标记。分析显示，除一个分离株外，所有分离株都属于最近描述的物种C。ocimiof the C.破坏物种复合体只有一个分离物被鉴定为C。严格意义上的破坏性（s.s.）在甜罗勒的致病性试验中，两者均为C。罗勒米安C. Destructivum s.s.分离株引发了黑斑病的典型症状，表明尽管如此。在该罗勒产区，罗勒属植物占优势，但它不是北方意大利黑斑病的唯一致病因子。而C. ocimiare已知世界各地，密切相关的speciesC. destructivum具有广泛的寄主范围，表明C. ocimi在这个物种复杂的驱动下适应主机。
Black spot is a major foliar disease of sweet basil (Ocimum basilicum) present in a typical cultivation area of northern Italy, including the Liguria and southern Piedmont regions, where this aromatic herb is an economically important crop. In this study, 15Colletotrichumisolates obtained from sweet basil plants with symptoms of black spot sampled in this area were characterized morphologically and by nuclear DNA analysis using internal transcribed spacers (ITS) and intervening 5.8S nrDNA as well as part of the beta-tubulin gene (TUB2) regions as barcode markers. Analysis revealed all but one isolate belonged to the recently described speciesC. ocimiof theC. destructivumspecies complex. Only one isolate was identified asC. destructivum sensu stricto(s.s.). In pathogenicity tests on sweet basil, bothC. ocimiandC. destructivum s.s.isolates incited typical symptoms of black spot, showing that althoughC. ocimiprevails in this basil production area, it is not the sole causal agent of black spot in northern Italy. While no other hosts ofC. ocimiare known worldwide, the close related speciesC. destructivumhas a broad host range, suggesting a speciation process ofC. ocimiwithin this species complex driven by adaptation to the host.