Complete genome sequences of twelve strains of Leptospira interrogans isolated from humans in Sri Lanka.
Complete genome sequences of twelve strains of Leptospira interrogans isolated from humans in Sri Lanka.
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DOI：
10.1016/j.meegid.2023.105462
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发表时间：
2023-06
期刊：
影响因子：
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通讯作者：
I. Senavirathna;D. Jayasundara;J. Warnasekara;Michael A. Matthias;J. Vinetz;S. Agampodi
中科院分区：
文献类型：
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作者：
I. Senavirathna;D. Jayasundara;J. Warnasekara;Michael A. Matthias;J. Vinetz;S. Agampodi
钩端螺旋体病（Leptospirosis）是由致病性钩端螺旋体（Leptospirosp.）被全球公认为一种新出现的人畜共患病。全基因组测序揭示了钩端螺旋体spathogenesis的隐藏信息。我们使用单分子实时（SMRT）测序获得了来自斯里兰卡发热患者的12个问号钩端螺旋体的全基因组序列，用于比较全基因组测序研究。序列数据产生了12个基因组，覆盖率大于X600，大小范围从4.62 Mb到5.16 Mb，G + C含量范围从35.00%到35.42%。NCBI（National Center for Biotechnology Information）基因组拼接平台预测的12株钩体编码序列总数在3845 ~ 4621个之间，在系统发育分析中，LPS生物合成位点大小相近且属于同一进化枝的钩体类群亲缘关系较近。尽管如此，编码糖生物合成的基因的变化被发现在血清型决定簇区域（rfblocus）。I型和III型CRISPR（重复的规则间隔短回文重复序列）系统在所有菌株中均被发现。这些序列的系统发育允许详细的基因组菌株分型。这些发现有助于我们更好地了解钩端螺旋体的发病机制，为钩端螺旋体的早期诊断、比较基因组分析和进化研究提供工具。
Leptospirosis, a major zoonotic disease caused by pathogenicLeptospiraspp. is recognized globally as an emerging zoonotic disease. Whole-genome sequencing reveals hidden messages aboutLeptospira'spathogenesis. We used Single Molecule Real-Time (SMRT) sequencing to obtain complete genome sequences of twelve L.interrogansisolates from febrile patients from Sri Lanka for a comparative whole genome sequencing study. The sequence data generated 12 genomes with a coverage greater than X600 with sizes ranging from 4.62 Mb to 5.16 Mb, and a G + C content ranging from 35.00% to 35.42%. The total number of coding sequences predicted by the NCBI (National Center for Biotechnology Information) genome assembly platform ranged from 3845 to 4621 for the twelve strains.Leptospiraserogroup with similar-sized LPS biosynthetic loci that belonged to the same clade had a close relationship in the phylogenetic analysis. Nonetheless, variations in the genes encoding sugar biosynthesis were found in the serovar determinant region(rfblocus). Type I and Type III CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) systems were found in all of the strains. Genome BLAST Distance Phylogeny of these sequences allowed for detailed genomic strain typing. These findings may help us better understand the pathogenesis, develop a tools for early diagnosis, comparative genomic analysis and evolution ofLeptospira.