Production of erythroid-potentiating activity by a human T-lymphoblast cell line.
Production of erythroid-potentiating activity by a human T-lymphoblast cell line.
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人 T 淋巴细胞系产生红细胞增强活性。
DOI：
10.1073/pnas.77.1.593
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发表时间：
1980
影响因子：
11.1
通讯作者：
A. Lusis
中科院分区：
文献类型：
--
作者：
D. Golde;N. Bersch;S. Quan;A. Lusis
我们衍生了一种人 T 淋巴细胞系 (Mo)，它可以组成型地产生某些淋巴因子。 Mo细胞产生人粒细胞-单核细胞前体体外生长所需的集落刺激因子，以及增强人红细胞祖细胞体外增殖的红细胞增强活性（EPA）。在血清存在的情况下，Mo 条件培养基中的 EPA 刺激小和大红细胞集落的生长几乎是原来的 2 倍。 EPA 也在无血清培养基中产生，并且当在人红系祖细胞的无血清培养物中进行测定时，它刺激集落生长约 3 倍。 Mo细胞系产生的EPA在体外不会刺激正常鼠类红细胞祖细胞（CFU-E）或Friend红白血病细胞的生长。 EPA 通过蛋白酶处理失活，但具有非常热稳定性，煮沸 15 分钟后大部分活性恢复。初步生化表征表明，EPA 是一种酸性糖蛋白，分子量约为 45,000。根据热稳定性和凝胶过滤色谱，EPA 显然可以与集落刺激因子分离。目前的观察结果为活化的 T 细胞产生对红细胞生成调节重要的体液因子这一概念提供了强有力的支持。产生人类 EPA 的细胞系的可用性应有助于蛋白质的表征，并允许对其生物效应进行明确的研究。
We derived a human T-lymphoblast cell line (Mo) that constitutively elaborates certain lymphokines. The Mo cells produce a colony-stimulating factor necessary for the growth of human granulocyte-monocyte precursors in vitro as well as an erythroid-potentiating activity (EPA) that enhances the proliferation of human erythroid progenitors in vitro. In the presence of serum, the EPA in Mo-conditioned medium stimulated the growth of small and large erythroid colonies almost 2-fold. EPA was also produced in serum-free medium, and, when assayed in serum-free cultures of human erythroid progenitors, it stimulated colony growth about 3-fold. The EPA produced by the Mo cell line did not stimulate normal murine erythroid progenitors (CFU-E) or Friend erythroleukemia cell growth in vitro. EPA was inactivated by protease treatment but was remarkably heat stable, with most of the activity recovered after boiling for 15 min. Preliminary biochemical characterization suggests that EPA is an acidic glycoprotein with molecular weight approximately 45,000. EPA is clearly separable from colony-stimulating factor on the basis of heat stability and gel-filtration chromatography. The present observations provide strong support for the concept that activated T cells produce humoral factors important in the regulation of erythropoiesis. The availability of a cell line producing human EPA should facilitate the characterization of the protein and permit definitive studies of its biologic effects.