Galactose Oxidase/Prussian Blue Based Biosensors
Galactose Oxidase/Prussian Blue Based Biosensors
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DOI：
10.1002/elan.201400720
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发表时间：
2015-06-01
期刊：
影响因子：
3
通讯作者：
Mousty, Christine
中科院分区：
文献类型：
--
作者：
Charmantray, Franck;Touisni, Nadia;Mousty, Christine
本文介绍了一种基于半乳糖氧化酶（GAOx）固定在锂皂石粘土膜上的电流型生物传感器的发展，该膜沉积在电沉积普鲁士蓝修饰的碳丝网印刷电极（PPD/PB/CSPE）上。使用几种GAOx基底测定GAOx@laponite/PPD/PB/CSPE生物电极的安培性能。使用这些修饰电极，在相对于Ag-AgCl的-0.2V下进行酶促产生的过氧化氢的还原。在最初的尝试中，使用双酶GAOx-TK生物传感器跟踪其固定形式上的大肠杆菌转酮醇酶活性。
This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen-Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly-(O-phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at -0.2V vs. Ag-AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx-TK biosensor.