Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy
Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy
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DOI：
10.1016/j.optcom.2009.10.032
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发表时间：
2010-02-01
影响因子：
2.4
通讯作者：
Sakai, Makoto
中科院分区：
文献类型：
--
作者：
Bokor, Nandor;Inoue, Keiichi;Sakai, Makoto
提出了一种基于瞬态荧光红外光谱饱和特性的扫描可见光超分辨显微镜。采用高斯红外光束、高斯可见光光束和拉盖尔-高斯（LG）可见光光束分别获得样品的双色激发荧光（2CF）图像。最终图像作为两个记录图像之间的差异获得。如果LG光束的峰值强度足够高以诱导荧光信号饱和，则原则上图像可以具有无限的空间分辨率。在目前的实验装置中，在可见光衍射极限（并且远远超过IR衍射极限）上的横向分辨率的类似于3倍的改进是容易实现的。(C)2009 Elsevier B. V.保留所有权利。
A scanning visible-super-resolution microscope based on the saturation behaviour of transient fluorescence detected infrared (TFD-IR) spectroscopy is proposed. A Gaussian IR beam, a Gaussian visible beam and a Laguerre-Gaussian (LG) visible beam are Used to obtain two separate two-color excitation fluorescence (2CF) images of the sample. The final image is obtained as the difference between the two recorded images. If the peak intensity of the LG beam is high enough to induce saturation in the fluorescence signal, the image can, in principle, have unlimited spatial resolution. A similar to 3-fold improvement in transverse resolution over the visible diffraction limit (and far exceeding the IR diffraction limit) is easily achievable in present experimental setups. (C) 2009 Elsevier B.V. All rights reserved.