Using PCR to Target Misconceptions about Gene Expression †
Using PCR to Target Misconceptions about Gene Expression †
复制标题
使用 PCR 消除有关基因表达的误解 †
DOI：
10.1128/jmbe.v14i1.539
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Dina L. Newman
中科院分区：
文献类型：
--
作者：
L. K. Wright;Dina L. Newman
我们提出了一个基于聚合酶链反应的实验室练习，可以用于一年级或二年级的生物学学生，以帮助克服关于基因表达的常见误解。生物学专业的学生通常不清楚基因（DNA）和基因表达（mRNA/蛋白质）之间的区别，并且通常认为基因只有在表达时才存在于生物体或细胞中。这个实验练习允许学生进行一个基于pcr的实验，旨在挑战他们对基因和基因表达之间差异的误解。学生首先用含有质粒的可诱导的GFP基因转化大肠杆菌，观察诱导和未诱导的菌落。当实际的PCR结果与他们在转化细胞中存在GFP基因的最初（不正确的）预测相矛盾时，下面的练习会产生认知失调。这个实验室练习的实地测试结果是在与基因和基因表达相关的概念的知识和应用问题上获得了学习收益。
We present a PCR-based laboratory exercise that can be used with first- or second-year biology students to help overcome common misconceptions about gene expression. Biology students typically do not have a clear understanding of the difference between genes (DNA) and gene expression (mRNA/protein) and often believe that genes exist in an organism or cell only when they are expressed. This laboratory exercise allows students to carry out a PCR-based experiment designed to challenge their misunderstanding of the difference between genes and gene expression. Students first transform E. coli with an inducible GFP gene containing plasmid and observe induced and un-induced colonies. The following exercise creates cognitive dissonance when actual PCR results contradict their initial (incorrect) predictions of the presence of the GFP gene in transformed cells. Field testing of this laboratory exercise resulted in learning gains on both knowledge and application questions on concepts related to genes and gene expression.
影响因子：
3.3
作者：
Schwartz, DL;Bransford, JD
通讯作者：
Bransford, JD