In vitro alachlor effects on reactive oxygen species generation, motility patterns and apoptosis markers in human spermatozoa
In vitro alachlor effects on reactive oxygen species generation, motility patterns and apoptosis markers in human spermatozoa
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DOI：
10.1016/j.reprotox.2006.08.007
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Janny, Laurent
中科院分区：
文献类型：
--
作者：
Grizard, Genevieve;Ouchchane, Lemlih;Janny, Laurent
氯乙酰苯胺类除草剂alachlor[2-氯-2′，6′-二乙基- n -（甲氧基甲基）-乙酰苯胺]由于其广泛的生产和应用，需要对其毒性进行评价，以确定最小的影响。在这项研究中，我们使用计算机辅助精子分析仪（CASA）检测了甲草胺对人类精子运动的体外影响。我们还对活性氧（ROS）和细胞凋亡标志物进行了评估，以探讨甲草胺改变精子运动的机制。暴露于甲草胺（0、0.18、0.37、0.90和1.85 mM） 2 h后，精子活精子率和精子速度呈浓度和/或时间依赖性降低。最敏感的参数是运动速度、平均路径速度和平均直线速度。甲草胺（1.85 mM）诱导ROS生成增加。还观察到线粒体膜电位（δ Psi(m)）降低，磷脂酰丝氨酸（PS）外化和DNA片段化增加，这是浓度和/或时间依赖性的。甲草胺的毒性作用可能导致氧化应激，这可能是细胞凋亡的中介。甲草胺也可能导致一些不孕病例。(c) 2006爱思唯尔公司版权所有。
Due to its extensive production and application, the toxicity of chloracetanilide herbicide alachlor[2-chloro-2',6'-diethyl-N-(methoxymethyl)-acetanilide] should be evaluated to establish minimum effects. In this study, we have examined the in vitro effects of alachlor on human sperm motion using a compunter-assisted sperm analyser (CASA). An evaluation of both reactive oxygen species (ROS) and markers of apoptosis was also performed to investigate the mechanism by which alachlor modifies the sperm movement.After exposure up to 2 h to alachlor (0, 0.18, 0.37, 0.90 and 1.85 mM), the percentage of viable, motile spermatozoa and sperm velocities were concentration and/or time dependently decreased. The most sensitive parameters were progressive motility, mean average path velocity and mean straight velocity. Alachlor (1.85 mM) induced an increase in ROS production. A decrease of mitochondrial membrane potential (Delta Psi(m)), an increase of both phosphatidylserine (PS) externalization and DNA fragmentation, which were concentration and/or time dependent, were also observed.It is possible that toxic effects of alachlor result in an oxidative stress which could act as a mediator of apoptosis. Alachlor could also contribute to some hypofertility cases. (c) 2006 Elsevier Inc. All rights reserved.