A histological assessment on the distribution of the osteocytic lacunar canalicular system using silver staining
A histological assessment on the distribution of the osteocytic lacunar canalicular system using silver staining
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DOI：
10.1007/s00774-007-0764-x
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发表时间：
2007-11-01
影响因子：
3.3
通讯作者：
Amizuka, Norio
中科院分区：
文献类型：
--
作者：
Hirose, Satoshi;Li, Minqi;Amizuka, Norio
考虑到骨重塑机制的复杂性以及必须绝对和谐才能完美发生的事件数量，骨细胞的时空分布及其腔隙小管系统可能影响骨重塑并受骨重塑影响的假设至少可以被认为是可行的。在这项研究中，我们使用舍恩银染法检查了发育中小鼠骨骼中骨细胞腔隙小管系统（OLCS）的分布。 3 日龄、2 周龄和 3 周龄小鼠的骨小梁显示出骨细胞的细胞质过程，但没有任何可察觉的排列。而且，许多肥厚的骨细胞无序地嵌入矿化骨基质中。然而，在 4 周龄时，小鼠骨骼显示出一些骨细胞突起，以直角到达骨表面，而其他骨细胞则显示出与 3 周龄标本中所见相同的特征。 8周龄的样本具有通常纺锤形的骨细胞，其组织方式与骨小梁的纵轴平行。它们还将细胞质突起垂直于骨表面延伸。然而，浸入较旧骨（即软骨和骨基质的残留混合物）中的一些骨细胞仍然显示出其细胞质过程的随机分布模式。到 12 周龄时，大多数骨细胞变得扁平，并且其长轴与骨表面平行。在皮质骨中也观察到了这种逐渐排列的趋势。我们进一步证明，8 周大的骨保护素缺乏的小鼠，其组织学证据显示骨转换高于平均水平，显示出 OLCS 紊乱。根据这项工作中收集的数据，随着正常骨重塑的进行，OLCS 似乎呈现出一种有组织的、可能与功能相关的空间分布。
Giving the complexity that characterizes the mechanisms of bone remodeling and the number of events that have to be in absolute harmony for it to occur flawlessly, the postulation that temporospatial distribution of osteocytes and their lacunar canalicular system might influence and be influenced by bone remodeling can be regarded, at least, as feasible. In this study, using Schoen's silver staining, we have examined the distribution of the osteocytic lacunar canalicular system (OLCS) in bones of developing mice. Trabecular bones of 3-day-old, 2-week-old, and 3-week-old mice displayed osteocytic cytoplasmic processes without any perceptible alignment. Also, many plump osteocytes were embedded in the mineralized bone matrix in a disorderly manner. At 4 weeks of age, however, mice bones showed some osteocytic processes that reached the bone surface on a right angle, while other osteocytes displayed the same features seen on 3-week specimens. Samples at 8 weeks of age featured osteocytes with their usual spindle shape, organized so as to parallel the longitudinal axis of trabecular bone. They also extended their cytoplasmic processes perpendicularly to the bone surface. However, several osteocytes immersed in older bone, i.e., a residual mix of cartilage and bone matrices, still showed a random pattern of distribution of their cytoplasmic processes. Up to 12 weeks of age, the majority of the osteocytes became flattened and were shown to be aligned with their long axis paralleling the bone surface. This tendency for such a gradual arrangement was also observed in cortical bones. We have further demonstrated that 8-week-old osteoprotegerin-deficient mice, which demonstrated histological evidence of higher than average bone turnover, revealed a disorganized OLCS. Given the data gathered in this work, the OLCS appears to assume an organized, probably function-related spatial distribution as normal bone remodeling goes on.