Efficacy of Salmonella enteritidis-immune lymphokines on horizontal transmission of S-arizonae in turkeys and S-gallinarum in chickens
Efficacy of Salmonella enteritidis-immune lymphokines on horizontal transmission of S-arizonae in turkeys and S-gallinarum in chickens
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DOI：
10.1016/s0168-1605(99)00036-7
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发表时间：
1999-05-01
影响因子：
5.4
通讯作者：
Kogut, MH
中科院分区：
文献类型：
--
作者：
Lowry, VK;Tellez, GI;Kogut, MH
亚利桑那沙门氏菌（SA）和鸡沙门氏菌（SG）对国际家禽生产具有重要的经济意义，因为它们在雏鸡孵化后的第一周发病。本实验室先前的研究表明，经肠炎沙门氏菌（SE）免疫的鸡产生的免疫淋巴因子（ILK）可保护日龄鸡和火鸡肉免受SE器官侵袭。以往的研究也表明，给肉仔鸡注射ILK可显著降低SG器官的侵袭。本研究的目的是确定ILK对SA在火鸡家禽和SG在肉鸡中水平传播发生率的影响。采用播种机/接触模型，评估了ILK给药对SA在禽类和SG在雏鸡中水平传播的影响。播种者被适当的细菌（SA火鸡，SG小鸡）攻击，接触者要么未经处理，要么施用ILK。在整个实验过程中，播种者和接触者同住一个实验组。测定了水平传播引起的死亡率和器官侵袭。未处理和ilk处理接触家禽之间的死亡率没有显著差异。相反，SG对肉鸡幼鸡的致病性极高。未处理的接触雏鸡死亡率约为68%，而ILK处理的接触雏鸡死亡率显著降低（P < 0.05）（15%）。免疫预防注射ILK可显著降低SA在接触性火鸡和SG在接触性肉鸡中的水平传播（P < 0.05）。与未处理的接触性家禽和雏鸡相比，经ILK处理的接触性雏鸡的肝/脾和盲肠扁桃体中SA和SC的细菌回收率显著降低。本研究结果强烈提示，雏鸡和肉鸡接种se免疫淋巴因子可显著降低沙门氏菌在家禽中的水平传播。这些结果提示了在家禽中使用非疫苗免疫预防策略来预防沙门氏菌的可能性。(C) 1999 Elsevier Science B.V.版权所有
Salmonella arizonae (SA) and S. gallinarum (SG) are of economic importance to international poultry production because of their pathogenesis in young poultry during the first week after hatching. Previous studies from our laboratory have shown immune lymphokines (ILK) produced by S. enteritidis (SE)-immunized chickens provide protection against SE organ invasion in day-old chickens and turkey poults. Previous studies have also demonstrated that SG organ invasion was significantly decreased by administration of ILK to broiler chicks. The objective of the present study was to determine the effect of ILK on the incidence of horizontal transmission of SA in turkey poults and of SG in broiler chicks. The effect of ILK administration on horizontal transmission of SA in poults and SG in chicks was assessed in a seeder/contact model. Seeders were challenged with the appropriate bacterium (SA turkeys, SG chicks), contacts were either untreated or administered ILK. Seeders and contacts cohabited within an experimental group throughout the experiment. Mortality and organ invasion as a result of horizontal transmission were determined. There were no significant differences in mortality between non-treated and ILK-treated contact poults. In contrast, SG was extremely pathogenic to young broiler chicks. Non-treated contact chicks had a mortality rate of approximately 68% whereas significant (P < 0.05) reduction in mortality was demonstrated in the contact chicks treated with ILK (15%). Horizontal transmission, as determined by organ invasion, of SA to contact turkey poults and SG to contact broiler chicks was also significantly (P < 0.05) decreased by immunoprophylactic administration of ILK. Bacterial recovery of SA from the liver/spleen and the cecal tonsil in contact poults and SC from contact chicks treated with ILK was dramatically reduced when compared to non-treated contact poults and chicks. Our results strongly suggest the immunoprophylactic administration of SE-immune lymphokines to young turkey poults and broiler chicks significantly reduces the horizontal transmission of Salmonella in poultry. These results suggest the possibilities of using a non-vaccine immunologically-based preventative strategy against Salmonella in poultry. (C) 1999 Elsevier Science B.V. All rights reserved.