Ciprofloxacin- and azithromycin-resistant bacteria in a wastewater treatment plant.
Ciprofloxacin- and azithromycin-resistant bacteria in a wastewater treatment plant.
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废水处理厂中的环丙沙星和阿奇霉素耐药细菌。
DOI：
10.1080/15459624.2023.2205485
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发表时间：
2023
影响因子：
2
通讯作者：
Reponen,Tiina
中科院分区：
文献类型：
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作者：
Niang,Mamadou;Reichard,JohnF;Maier,Andrew;Talaska,Glenn;Ying,Jun;SantoDomingo,Jorge;Varughese,Eunice;Boczek,Laura;Huff,Emma;Reponen,Tiina
污水处理厂中抗药性细菌的出现已成为一个职业和环境问题。污水处理厂是一种工程系统，在排放到环境中之前处理废水以满足公共卫生标准。然后，作为流出物或固体的残余物被排放或有益地再循环到环境中。由于这些废物含有各种各样的微生物，其中一些对常用的抗生素有抗药性，这些微生物有可能通过残余物的回收和污水排放在环境中传播。人类感染ARB的情况正在增加，目前尚不清楚人与环境之间的相互作用在这一过程中如何发挥作用。在第一线工作的污水处理厂工人可能会直接接触到含有这些微生物的材料。本研究的目的是确定ARB的数量存在于空气和污水污泥中的污水处理厂使用非选择性培养基补充两种抗生素（环丙沙星和阿奇霉素）。总异养菌、环丙沙星耐药菌和阿奇霉素耐药菌的密度分别为7.82 × 105 - 4.7 × 109、7.87 × 103 - 1.05 × 108和2.27 × 105 - 1.16 × 109 CFU/g。经处理的污泥中环丙沙星耐药菌的流行率[（含抗生素培养基上的浓度/不含抗生素培养基上的浓度）× 100]比消化污泥低两倍，比原污泥低约三倍。对于阿奇霉素，耐药菌在处理污泥中的流行率与消化污泥大致相同，比原料污泥低近两倍。尽管两种抗生素的脱水处理污泥中耐药细菌的平均患病率显着降低，但这些差异并不显着。观察到阿奇霉素的抗生素耐药性发生率最高。同样，带式压滤机室（BFPR）内空气中阿奇霉素耐药菌的流行率几乎是空气中环丙沙星耐药菌流行率的7倍。这些浓度的ARB是不可忽略的，可能代表了一些工人在污水处理厂的接触途径。
The occurrence of antibiotic-resistant bacteria (ARB) in wastewater treatment plants (WWTPs) has become an occupational and environmental concern. WWTPs are engineered systems that treat wastewater to meet public health standards before release into the environment. The residuals, as either effluent or solids, are then discharged or beneficially recycled into the environment. Since these wastes contain a diverse array of microorganisms, some of which are resistant to commonly used antibiotics, there is a potential for these organisms to spread in the environment via residual recycling and effluent discharge. Human infections with ARB are increasing, and it is not well known how the interaction between humans and the environment plays a role in this process. WWTP workers, who are on the front lines, may come into direct contact with materials containing these microbes. This study aimed to determine the number of ARB present in both air and sewage sludges in a WWTP using nonselective media supplemented with two antibiotics (ciprofloxacin and azithromycin). The densities of total heterotrophic bacteria, ciprofloxacin-resistant bacteria, and azithromycin-resistant bacteria were 7.82 × 105− 4.7 × 109, 7.87 × 103− 1.05 × 108, and 2.27 × 105− 1.16 × 109CFU/g, respectively. The prevalence [(concentration on medium with antibiotics/concentration on medium without antibiotics) × 100] of ciprofloxacin-resistant bacteria in treated sludge was twice as low as in digested sludge and approximately three times lower than in raw sludge. For azithromycin, the prevalence of resistant bacteria in treated sludge was about the same in digested and nearly twice lower than in raw sludge. Despite a marked reduction in the mean prevalence of resistant bacteria in dewatered treated sludge for both antibiotics, these differences were not significant. The highest prevalence of antibiotic resistance was observed for azithromycin. Similarly, the prevalence of airborne azithromycin-resistant bacteria inside the belt filter press room (BFPR) was nearly seven times higher than the prevalence of airborne ciprofloxacin-resistant bacteria. These concentrations of ARB were not negligible and may represent an exposure pathway for some workers in WWTPs.