Boron concentration measurements by alpha spectrometry and quantitative neutron autoradiography in cells and tissues treated with different boronated formulations and administration protocols
Boron concentration measurements by alpha spectrometry and quantitative neutron autoradiography in cells and tissues treated with different boronated formulations and administration protocols
复制标题
DOI：
10.1016/j.apradiso.2013.12.001
复制
发表时间：
2014-06-01
影响因子：
1.6
通讯作者：
Altieri, Saverio
中科院分区：
文献类型：
--
作者：
Bortolussi, Silva;Ciani, Laura;Altieri, Saverio
高精度测量受照射组织中的硼浓度的可能性是安全有效的BNCT治疗的基本步骤。在帕维亚，两种技术被用于这一目的，一种是基于带电粒子光谱的定量方法，另一种是基于中子放射自显影的硼生物分布成像。在BNCT可行性研究的框架内，最近建立了一种用中子放射自显影测定硼浓度的定量方法，并将其用于生物样品的测量，包括固体和液体样品。对这项技术进行了校准，并将获得的结果与光谱分析的结果进行了交叉验证，以验证它们。这些比较是使用从动物身上提取的组织进行的，这些组织接受了不同的硼管理方案。随后，用定量中子放射自显影技术测量了用双酚A和新的硼化制剂处理的骨肉瘤细胞样本。(C)2013爱思唯尔有限公司。保留所有权利。
The possibility to measure boron concentration with high precision in tissues that will be irradiated represents a fundamental step for a safe and effective BNCT treatment. In Pavia, two techniques have been used for this purpose, a quantitative method based on charged particles spectrometry and a boron biodistribution imaging based on neutron autoradiography. A quantitative method to determine boron concentration by neutron autoradiography has been recently set-up and calibrated for the measurement of biological samples, both solid and liquid, in the frame of the feasibility study of BNCT. This technique was calibrated and the obtained results were cross checked with those of a spectrometry, in order to validate them. The comparisons were performed using tissues taken form animals treated with different boron administration protocols. Subsequently the quantitative neutron autoradiography was employed to measure osteosarcoma cell samples treated with BPA and with new boronated formulations. (C) 2013 Elsevier Ltd. All rights reserved.