Serum immunoreactive leptin concentrations in normal-weight and obese humans
Serum immunoreactive leptin concentrations in normal-weight and obese humans
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DOI：
10.1056/nejm199602013340503
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发表时间：
1996-02-01
影响因子：
158.5
通讯作者：
Caro, JF
中科院分区：
文献类型：
--
作者：
Considine, RV;Sinha, MK;Caro, JF
背景。瘦素是ob基因的产物，是脂肪细胞分泌的一种激素，ob基因突变的动物肥胖，给予瘦素后体重减轻，但对瘦素在人体中的生理作用知之甚少。使用新开发的放射免疫测定法，我们测量了136名正常体重受试者和139名肥胖受试者的血清瘦素浓度（身体质量指数，男性大于等于27.3，女性大于等于27.8；身体质量指数定义为体重（公斤）除以身高（米）的平方）。7名肥胖受试者在体重减轻后和维持较低体重期间重复了这些测量。测定了27例正常体重者和27例肥胖者脂肪细胞mRNA的含量。肥胖受试者的平均（+/-SD）血清瘦素浓度为31.3+/-24.1 ng / ml，正常体重受试者的平均（+/-SD）血清瘦素浓度为7.5+/-9.3 ng / ml （P < 0.001）。血清瘦素浓度与体脂率呈正相关（r = 0.85, P < 0.001）。肥胖受试者的脂肪细胞ob mRNA含量约为正常体重受试者的两倍（P = 0.005），并与54名受试者的体脂百分比相关（r = 0.68, P < 0.001）。在减肥后的7名肥胖受试者中，血清瘦素浓度和脂肪细胞ob mRNA含量均下降，但在维持较低体重期间，这些指标再次升高。血清瘦素浓度与体脂百分比相关，这表明大多数肥胖者对内源性瘦素的产生不敏感。(C) 1996年，马萨诸塞州医学会。
Background. Leptin, the product of the ob gene, is a hormone secreted by adipocytes, Animals with mutations in the ob gene are obese and lose weight when given leptin, but little is known about the physiologic actions of leptin in humans.Methods. Using a newly developed radioimmunoassay, we measured serum concentrations of leptin in 136 normal-weight subjects and 139 obese subjects (body-mass index, greater than or equal to 27.3 for men and greater than or equal to 27.8 for women; the body-mass index was defined as the weight in kilograms divided by the square of the height in meters). The measurements were repeated in seven obese subjects after weight loss and during maintenance of the lower weight. The ob messenger RNA (mRNA) content of adipocytes was determined in 27 normal-weight and 27 obese subjects.Results. The mean (+/-SD) serum leptin concentrations were 31.3+/-24.1 ng per milliliter in the obese subjects and 7.5+/-9.3 ng per milliliter in the normal-weight subjects (P < 0.001). There was a strong positive correlation between serum leptin concentrations and the percentage of body fat (r = 0.85, P < 0.001). The ob mRNA content of adipocytes was about twice as high in the obese subjects as in the normal-weight subjects (P = 0.005) and was correlated with the percentage of body fat (r = 0.68, P < 0.001) in the 54 subjects in whom it was measured. In the seven obese subjects studied after weight loss, both serum leptin concentrations and ob mRNA content of adipocytes declined, but these measures increased again during the maintenance of the lower weight.Conclusions. Serum leptin concentrations are correlated with the percentage of body fat, suggesting that most obese persons are insensitive to endogenous leptin production. (C) 1996, Massachusetts Medical Society.