Effect of nonsurgical periodontal therapy on crevicular fluid levels of Cathepsin K in periodontitis
Effect of nonsurgical periodontal therapy on crevicular fluid levels of Cathepsin K in periodontitis
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DOI：
10.1016/j.archoralbio.2009.08.007
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发表时间：
2009-11-01
影响因子：
3
通讯作者：
Thorat, Manoj Kumar
中科院分区：
文献类型：
--
作者：
Garg, Garima;Pradeep, A. R.;Thorat, Manoj Kumar
目的：组织蛋白酶K （CTSK）主要在破骨细胞中表达，是一种有效的细胞外基质降解酶，在破骨细胞介导的骨吸收中起关键作用。它的牙龈沟液（GCF）水平增加在牙周病已报道在以前的研究。本研究旨在评估CTSK在牙周病中的作用，并确定牙周治疗对GCF中CTSK浓度的影响。设计：60名受试者根据牙龈指数（GI）、探诊袋深度（PPD）和临床附着丧失（CAL）分为健康（I组）、牙龈炎（II组）和慢性牙周炎（III组）3组（n = 20）。第四组（M组）由来自III组的20名受试者组成，在非手术牙周治疗（刮治和牙根刨平）后6-8周。采用ELISA对每位患者采集的GCF样本进行CTSK定量。结果：GCF中CTSK的平均浓度以ⅲ组最高，为55.55 pmol/l。ⅰ组和ⅱ组GCF中CTSK平均浓度分别为5.95 pmol/l和6.90 pmol/l。IV组GCF中CTSK的平均浓度降至11.15 pmol/l，略高于I和II组。结论：牙周炎患者GCF CTSK水平升高，且与GI、PPD、CAL等临床参数呈负相关。牙周炎非手术治疗后CTSK水平降低。因此，CTSK可以被认为是牙周病的“破骨细胞活性标志物”，也值得进一步考虑作为治疗靶点。2009爱思唯尔有限公司版权所有。
Objectives: Cathepsin K (CTSK), predominantly expressed in osteoclasts, is a potent extracellular matrix degrading enzyme that plays a critical role in osteoclast-mediated bone resorption. Its increased gingival crevicular fluid (GCF) levels in periodontal disease have been reported in a previous study. The present study has been carried out to assess the role of CTSK in periodontal disease and to determine the effect of periodontal treatment on CTSK concentration in GCF.Design: 60 subjects were divided into three groups (n = 20) based on gingival index (GI), probing pocket depth (PPD) and clinical attachment loss (CAL): healthy (group I), gingivitis (group II) and chronic periodontitis (group III). A fourth group (group M consisted of 20 subjects from group III, 6-8 weeks after nonsurgical periodontal therapy (scaling and root planing). GCF samples collected from each patient were quantified for CTSK using ELISA.Results: The mean CTSK concentration in GCF was found to be the highest in group III, i.e. 55.55 pmol/l. The mean CTSK concentration in GCF in group I and group II was 5.95 pmol/l and 6.90 pmol/l respectively. The mean CTSK concentration in GCF in group IV decreased to 11.15 pmol/l, slightly more than that in groups I and II.Conclusions: GCF CTSK levels increased in periodontitis and correlated negatively with clinical parameters like GI, PPD and CAL. CTSK levels decreased after nonsurgical treatment of periodontitis. Thus, CTSK can be considered as a 'marker of osteoclastic activity' in periodontal disease and also deserves further consideration as a therapeutic target. (C) 2009 Elsevier Ltd. All rights reserved.