MDA-7/IL-24 suppresses human ovarian carcinoma growth in vitro and in vivo.
MDA-7/IL-24 suppresses human ovarian carcinoma growth in vitro and in vivo.
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DOI：
10.1186/1476-4598-6-11
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发表时间：
2007-02-02
期刊：
影响因子：
37.3
通讯作者：
Ramesh R
中科院分区：
文献类型：
--
作者：
Gopalan B;Shanker M;Chada S;Ramesh R
以往的研究表明，人黑色素瘤分化相关基因-7(MDA-7)，又称白介素24(IL-24)，对人和小鼠癌细胞具有很强的抗肿瘤活性。然而，这些研究中的大多数仅限于体外试验。在本研究中，我们研究了MDA-7/IL-24在体外和体内对人卵巢癌细胞的抗肿瘤活性。在体外，携带MDA-7基因的腺病毒载体(Ad-MDA7)处理卵巢癌细胞后，可抑制细胞增殖，诱导细胞周期停滞，导致细胞凋亡。在Ad-MDA7处理的正常细胞中，我们没有观察到抑制活性。在体内，与对照组相比，Ad-MDA7治疗皮下移植瘤可显著抑制肿瘤生长(p<；0.001)。对经Ad-MDA7处理的卵巢肿瘤组织裂解物的分子分析表明，MDA-7蛋白的表达与caspase级联反应的激活有关。我们的结果表明，无论是在体外还是在体内，MDA-7/IL-24处理卵巢癌细胞都会导致生长抑制。
Previous studies showed that the human melanoma differentiation-associated gene-7 (mda-7), also known as interleukin-24 (IL-24), has potent antitumor activity against human and murine cancer cells. However, the majority of these studies were limited to in vitro testing. In the present study, we investigated the antitumor activity of mda-7/IL-24 against human ovarian cancer cells both in vitro and in vivo. In vitro, treatment of ovarian cancer cells with an adenoviral vector carrying the mda-7 gene (Ad-mda7) resulted in inhibition of cell proliferation and induction of cell cycle arrest, leading to apoptosis. We did not observe inhibitory activity in Ad-mda7-treated normal cells. In vivo, treatment of subcutaneous tumor xenografts with Ad-mda7 resulted in significant tumor growth inhibition when compared with that in control groups (p < 0.001). Molecular analysis of ovarian tumor tissue lysates treated with Ad-mda7 showed that MDA-7 protein expression was associated with activation of the caspase cascade. Our results show that treatment of ovarian cancer cells with mda-7/IL-24 results in growth suppression both in vitro and in vivo.
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DOI：
10.1073/pnas.152327199
发表时间：
2002-07-23
影响因子：
11.1
作者：
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影响因子：
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