Diverse Amino Acid Changes at Specific Positions in the N-Terminal Region of the Coat Protein Allow Plum pox virus to Adapt to New Hosts
Diverse Amino Acid Changes at Specific Positions in the N-Terminal Region of the Coat Protein Allow Plum pox virus to Adapt to New Hosts
复制标题
DOI：
10.1094/mpmi-04-13-0093-r
复制
发表时间：
2013-10-01
影响因子：
3.5
通讯作者：
Simon-Mateo, Carmen
中科院分区：
文献类型：
--
作者：
Carbonell, Alberto;Maliogka, Varvara I.;Simon-Mateo, Carmen
李痘病毒（PPV）-D和PPV-R是从PPV D株分离的两个分离株，它们的宿主特异性不同。先前对PPV-R和PPV-D嵌合体的分析表明，外壳蛋白（CP）的N末端包括宿主特异性致病性决定簇。在此，通过分析在PPV-R骨架中含有PPV-D 3'区片段（包括编码CP的区域）的嵌合体的草本和木本物种中的感染性，精确地绘制了这些决定因素。这些嵌合体在桃中不具侵染性，但能系统侵染烟草和烟草。当CP的N末端的短的30个氨基酸区域中的特定氨基酸被修饰或缺失时，本塞姆氏菌是本塞姆氏菌。大多数这些突变并没有减少PPV健身李属。尽管其他的则削弱了该宿主的全身感染。我们提出了一个模型，在该模型中，CP的N末端，高度相关的病毒系统运动，是有针对性的宿主防御机制在烟草属。在这个短区域的突变允许PPV克服在这个主机的防御反应，但可以妥协的效率PPV系统运动在其他主机，如李属。
Plum pox virus (PPV)-D and PPV-R are two isolates from strain D of PPV that differ in host specificity. Previous analyses of chimeras originating from PPV-R and PPV-D suggested that the N terminus of the coat protein (CP) includes host-specific pathogenicity determinants. Here, these determinants were mapped precisely by analyzing the infectivity in herbaceous and woody species of chimeras containing a fragment of the 3' region of PPV-D (including the region coding for the CP) in a PPV-R backbone. These chimeras were not infectious in Prunus persica, but systemically infected Nicotiazza clevelandii and N. benthamiana when specific amino acids were modified or deleted in a short 30-amino-acid region of the N terminus of the CP. Most of these mutations did not reduce PPV fitness in Prunus spp. although others impaired systemic infection in this host. We propose a model in which the N terminus of the CP, highly relevant for virus systemic movement, is targeted by a host defense mechanism in Nicotiana spp. Mutations in this short region allow PPV to overcome the defense response in this host but can compromise the efficiency of PPV systemic movement in other hosts such as Prunus spp.