Sec15, a component of the exocyst, promotes notch signaling during the asymmetric division of Drosophila sensory organ precursors
Sec15, a component of the exocyst, promotes notch signaling during the asymmetric division of Drosophila sensory organ precursors
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DOI：
10.1016/j.devcel.2005.06.010
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发表时间：
2005-09-01
影响因子：
11.8
通讯作者：
Bellen, HJ
中科院分区：
文献类型：
--
作者：
Jafar-Nejad, H;Andrews, HK;Bellen, HJ
果蝇感觉器官前体（SOPs）的不对称分裂产生成熟感觉器官的不同细胞类型。在一项旨在鉴定这一过程中新型参与者的遗传筛选中，我们分离出了果蝇sec15的一个突变，该基因编码胞吐复合体的一个组分，胞吐复合体是一种在进化上保守的、与细胞内囊泡运输有关的复合物。sec15缺失的感觉器官含有额外的神经元，而支持细胞减少，这种表型与Notch信号缺失一致。一个包含Notch、Sanpodo和内吞的Delta的囊泡区室在突变的SOPs的基部区域积累。基于Sec15的动态运输、它与再循环内体标记物Rab11的共定位以及Rab11在sec15克隆中的异常分布，我们提出Delta再循环的缺陷导致sec15缺失的感觉细胞谱系中的细胞命运转变。我们的数据表明Sec15介导一种特定的囊泡运输事件，以确保果蝇中正确的神经元命运特化。
Asymmetric division of sensory organ precursors (SOPS) in Drosophila generates different cell types of the mature sensory organ. In a genetic screen designed to identify novel players in this process, we have isolated a mutation in Drosophila sec15, which encodes a component of the exocyst, an evolutionarily conserved complex implicated in intracellular vesicle transport. sec15(-) sensory organs contain extra neurons at the expense of support cells, a phenotype consistent with loss of Notch signaling. A vesicular compartment containing Notch, Sanpodo, and endocytosed Delta accumulates in basal areas of mutant SOPS. Based on the dynamic traffic of Sec15, its colocalization with the recycling endosomal marker Rab11, and the aberrant distribution of Rab11 in sec15 clones, we propose that a defect in Delta recycling causes cell fate transformation in sec15- sensory lineages. Our data indicate that Sec15 mediates a specific vesicle trafficking event to ensure proper neuronal fate specification in Drosophila.