Detection of HIV-1 in Saliva: Implications for Case-Identification, Clinical Monitoring and Surveillance for Drug Resistance.
Detection of HIV-1 in Saliva: Implications for Case-Identification, Clinical Monitoring and Surveillance for Drug Resistance.
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DOI：
10.2174/1874357901004010088
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Klausner, Jeffrey D
中科院分区：
文献类型：
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作者：
Balamane, Maya;Winters, Mark A;Klausner, Jeffrey D
背景：检测抗体的唾液测试用于诊断HIV感染。本研究的目的是确定唾液是否可以用于核酸检测，以测量HIV-1病毒载量（VL）和检测耐药性。方法：69例HIV感染者提供5-10 ml的唾液和血液样本。使用Nuclisens提取法从唾液和干血斑中分离病毒RNA。使用改良的Amplicor测定法测量唾液VL，并使用内部RT-PCR/测序方案进行基因分型。血浆VL同时提请临床tests.RESULTS：36 47（77%）血浆病毒血症患者有可测量的唾液HIV-1 RNA。配对的血浆和唾液HIV RNA水平显著相关（斯皮尔曼相关系数= 0.6532，p
BACKGROUND: Saliva tests that detect antibodies are used to diagnose HIV infection. The goal of this study was to determine whether saliva could be used for nucleic acid-based tests to measure HIV-1 virus load (VL) and detect drug resistance.METHODS: 69 HIV infected individuals provided 5-10 ml of saliva and blood samples. Viral RNA was isolated from saliva and dried blood spots using the Nuclisens extraction. Saliva VL was measured using a modified Amplicor assay, and genotyping was performed using an in-house RT-PCR/sequencing protocol. Plasma VLs were obtained from concurrently drawn clinical tests.RESULTS: Thirty-six of 47 (77%) plasma viremic patients had measurable saliva HIV-1 RNA. Paired plasma and saliva HIV RNA levels were significantly correlated (Spearman's correlation = .6532, p